摘要
目的 研究慢性乙型肝炎患者外周血树突状细胞 (DC)的免疫功能。方法 从慢性乙型肝炎患者外周血中分离单个核细胞 ,用无血清培养法分离培养DC ,用流式细胞仪检测DC表型 ,用液闪计数仪观察DC对T细胞的增殖作用 ,用ELISA法检测混合淋巴细胞反应 (MLR)中细胞因子的分泌水平。结果 患者组DC的CD86的表达率为 (70 .2± 5 .2 ) % ,明显低于正常人组 (95 .3±3.8) % ,P <0 .0 1;其诱导T细胞增殖能力每分钟液闪计数cpm为 10 0 0 0± 2 0 0 0 ,明显低于正常人组(cpm为 30 0 0 0± 30 0 0 ) ,P <0 .0 1;患者MLR中IL 12为 (12 0 .0± 19.7) pg/ml,γ 干扰素为 (799.0± 16 1.3) pg/ml,明显低于正常人组的 (2 80 .0± 41.1) pg/ml和 (335 9.0± 6 35 .4) pg/ml,P <0 .0 1。 结论 慢性乙型肝炎患者外周血DC免疫功能低下 ,并与DC表面CD86的表达率下降及DC分泌IL
Objective To study immune function of peripheral blood dendritic cells in chronic hepa titis B virus infected patients. Methods Peripheral blood DCs of patients and normal human were isolated and cultured in serum free media. The expression levels of DC surface molecule were analyzed by flow cytometry and the ability of DC to induce T lymphocyte proliferation were evaluated by a liquid scintillation counter and the amounts of cytokines in MLR were measured detected. Results The expression rate of CD86 (70.2±5.2)% on DC in patients was decreased compared with that in controls. (95.3±3.5)%, P < 0.01. And the ability of DC in patients to induce T lymphocyte proliferation (cpm, 10 000±2 000) was significantly lower than that of controls (cpm, 30 000±3 000, P <0.01) and the amounts of cytokines in MLR of patients was less (IL 12, 120±19.7pg/ml; IFN γ,799±161.3 pg/ml) than that of controls (IL 12,280±41.1 pg/ml;IFN γ,3 359±635.4 pg/ml, P <0.01).Conclusions Immune function of peripheral blood DC in chronic hepatitis B virus infected patients is lower than that in normal human, which is closely related to the low expression level of CD86 on DC and the impaired capicity of producing cytokines.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2001年第3期144-147,共4页
Chinese Journal of Infectious Diseases
基金
国家自然科学基金资助项目 ( 395 70 6 5 5 )
