摘要
目的:探讨HBsAg脉冲的树突状细胞(DC),对细胞因子诱导的杀伤细胞(CIK)的增殖和杀伤作用的影响。方法:选择慢性乙肝患者23例,用常规方法分离外周血单个核细胞(PBMC),经HBsAg脉冲后培养为特异性的DC,用3H-TdR掺入法检测该细胞对CIK细胞增殖的刺激作用,用乳酸脱氢酶释放法检测特异性CIK细胞对HepG2215细胞的特异性杀伤作用。结果:HBsAg脉冲的DC对CIK细胞的增殖具有刺激作用。由HBsAg脉冲的DC所诱导的特异性CIK细胞对HepG2215细胞的特异性杀伤作用增强。结论:HBsAg脉冲的DC可增强CIK细胞的杀伤活性。
AIM： To explore the effects of HBsAg- pulsed dendritic cells （DCs） on the proliferation and killing functions of cytokine-induced killer （ CIK ） cells. METHODS： The peripheral blood mononuclear cells （PBMCs） were isolated from the patients with chronic hepatitis B by the routine method, and induced into specific DCs with HBsAg pulsed. The ^3H-TdR incorporation method was used to determine the stimulation effect of HBsAg-pulsed DCs on the proliferation of CIK cells. LDH release assay was used to measure the specific killing activity of CIK cells on HepG2215 cells. RESULTS： The HBsAg-pulsed DCs could induce the memory proliferation of CIK cells and strengthen the killing activity of CIK cells （P〈0.05）. CONCLUSION： HBsAg-pulsed DCs can enhance the proliferation and killing functions of CIK cells.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2005年第5期634-636,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
广东省重点科技资助项目(No.99MO4811G)
