摘要
目的 :了解喜树碱 (CPT)诱导鼻咽癌细胞凋亡是否与线粒体膜电位改变有关。方法 :用 2 μmol/ L 的CPT 处理 CNE- 2 Z细胞 ,经流式细胞仪、Hoechst332 5 8/ PI双重染色鉴定凋亡细胞 ,同时用罗丹明 12 3(Rhodamine12 3)染色后 ,经流式细胞仪检测线粒体膜电位。结果 :CPT可明显降低线粒体膜电位 ,2 μm ol/ L 的CPT处理 CNE- 2 Z细胞 2 4h后 ,罗丹明 12 3摄取量低的细胞明显增加 ,达 (19.0± 3.0 ) % ,与 DMSO对照组的(7.0± 1.4) %相比 ,差异有显著性意义 (P <0 .0 5 )。结论 :降低线粒体膜电位可能是 CPT诱导鼻咽癌细胞凋亡的机制之一。
Objective:To understand whether CPT induced apoptosis is related to the alteration of mitochondrial transmembrane potentials (△Ψ m).Method:A low diffirentiation huamn nasopharyngeal carcinoma (NPC) cell line of CNE 2Z cells were used as in vitro model.After treatment with 2 μmol/L CPT for 24 h, the △Ψ m was detected by the staining of rhodamine 123 (Rh123), while apoptosis was confirmed by sub G1 cell content and the double staining of hoechst33258 and propidium iodide (PI).Result:CPT induced △Ψ m collapse and apoptosis.With 2 μmol/L CPT treatment for 24 h, △Ψ m low cell was increased to ( 19.0 ± 3.0 )%, while in the DMSO control, △Ψ m low was ( 7.0 ± 1.4 )% P< 0.05 .Conclusion:The disruption of the △Ψ m may be one of the mechanisms of CPT induced apoptosis in CNE 2Z cells.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2001年第7期309-310,I005,共3页
Journal of Clinical Otorhinolaryngology
基金
广东省重点学科经费资助项目 (No.930 7)
