摘要
目的 研究有关生化因素对喜树碱（ＣＰＴ）诱导人鼻咽癌细胞凋亡的影响。方法 在ＣＰＴ诱导人鼻咽癌ＣＮＥ－２Ｚ 细胞凋亡的基础上，采用流式细胞仪及二苯胺反应等方法，观察了多胺、Ｃａ２＋、Ｍｇ２十依赖性内切酶活性抑制剂及ＰＡＲＰ活性抑 制剂对ＤＮＡ片段化形成的影响。结果 多胺可明显抑制ＣＰＴ诱导的ＣＮＥ－２Ｚ细胞凋亡，使片段化ＤＮＡ明显降低，Ｐ＜０．０５。内 切酶及ＰＡＲＰ活性抑制剂与ＣＰＴ共同作用ＣＮＥ－２Ｚ细胞后测得片段化ＤＮＡ与单用ＣＰＴ相比无显著性差异，Ｐ＞０．０５。结论 喜树碱诱导的 ＣＮＥ－２Ｚ细胞凋亡可能与一种非 Ｃａ２＋、Ｍｇ２＋依赖性内切酶活性有关，且受多胺的调节。
Objective To study the effects of some biochemical factors on apoptosis in nasopharyngeal carcinoma cells (CEN-2Z) induced by DNA topoisomerase I inhibitor camptothecin (CPT). Methods DNA fragmentation was analysed with diphenylamine reaction. Results Spermine could obviously reduce the DNA fragmentation in CHE-2Z cells beated with 4 mol/L CPT, P<0.05. Neither depletion of intracellular calcium by EGTA nor inhibiting the activity of an endonuclease or poly (ADP-ribose) polymerase could effect the DNA fragmentation in CNE-2Z cells treated with 4 mol/L CPT, P>0.05. Conclusion The above results suggested that a Ca2+.Mg2+ independent endonuclease might be involved in the apoptosis in CNE-2Z cells induced by DNA topoisomerase I inhibitor camptothecin.
出处
《中国实验诊断学》
2000年第5期204-205,共2页
Chinese Journal of Laboratory Diagnosis
基金
广东省重点学科经费资助
关键词
鼻咽癌
喜树碱
细胞凋亡
调节
生化因素
nasopharyngeal carcinoma
camptothecin: apoptosis, modulation
