摘要
目的 了解医院感染中高产 Am p Cβ-内酰胺酶阴沟肠杆菌的流行情况。方法 通过药敏实验和等电点分析筛选出高产 Am p Cβ-内酰胺酶的阴沟肠杆菌 ,然后通过诱导实验分析了其 Am p Cβ-内酰胺酶表型 ,最后 ,通过PFGE分型技术考察了其克隆构成情况。结果 在 35株阴沟肠杆菌中 ,2 8株 (80 % )高产 Am p Cβ-内酰胺酶 ,其中18株 (6 5 % )属完全去抑制型 ,10株 (35 % )属部分去抑制型 ;2 8株高产 Am p Cβ-内酰胺酶阴沟肠杆菌的染色体DNA指纹图呈 17种克隆的多克隆构成模式。结论 阴沟肠杆菌引起的医院感染中流行高产 Amp Cβ-内酰胺酶的菌株 ,完全去抑制型为其主要表型 ;其内源性感染方式使阴沟肠杆菌医院感染株呈多克隆构成模式。
OBJECTIVE A molceular epidemiology study of resistant Enterobacter cloacae which hyperproduced Amp Cβ lactamase was conducted to learn the prevailing situation in nosocomial infection. METHODS E.cloacae hyperproducing Amp Cβ lactamase was screened out by susceptibility test and isoelectric focusing (IEF). And the phenotype of Amp Cβ lactamase was identified by induced test. Finally pulsed field gel electrophoresis (PFGE) typing method was used to investigate the clonal constitution. RESULTS Thirty five strains of E.cloacae from the First Affliated Hospital of MSSU were analysed in 1998.1 1998.6, from which 28 strains (80%) hyperproduced Amp Cβ lactamase. Eighteen strains(65%) stably derepressed and 10(35%) partial derepressed strains were identified by induced test. A multiclone mould (17 clones) was found in 28 strains of E.cloacae. CONCLUSIONS Stably derepressed E.cloacae that hyperproduced Amp Cβ lactamase mainly prevailed in our hospital. The multiclonal constitution of E.cloacae from nosocomial infection which hyperproduced Amp Cβ lactamase was due to its endogenously acquired infection.
出处
《中华医院感染学杂志》
CAS
CSCD
2001年第4期254-257,共4页
Chinese Journal of Nosocomiology
基金
美国中华医学会临床微生物及感染性疾病项目资助
