摘要
为建立ＤＮＡ指纹技术在近交系大鼠遗传监测中的方法，采用ＤＮＡ指纹图对国内已知的７个品系近交系大鼠群体进行了分析，并对相同ＤＮＡ进行了多次ＤＮＡ指纹图重复实验，其结果表明：不同品系之间ＤＮＡ指纹图差异较大，其平均图带数为（１６．３６０±２．１７８），共有带率为（０．０６１±０．００８），相似系数为（０．０６２±０．００８），相同ＤＮＡ指纹图概率为３．６９１×１０－２３。相同ＤＮＡ不同次制作的ＤＮＡ指纹图谱基本一致（Ｐ＞０．０５）。同一个体不同组织的ＤＮＡ指纹图也基本相同，从而证实了该方法具有高度的稳定性和可重复性。
In order to set up the method of DNA fingerprints in th e heredity monitoring of inbred rat, national nine groups of seven strains were analysed using DNA fingerprints. and the same DNA sample were tested more times in DNA fingerprints. The result indicates：There were great diversity in various strain of DNA fingerprints, in which average bands were (16.360±2.178)，band sharing level was (0.061±0.008)，and the similarity coefficient was (0.062±0.008)， the probability of an identical DNA fingerprints in them were 3.691×10-23. D NA fingerprints from the same DNA were almost coincident by various times exper iment (P>0.05). and which from different organization DNA of the same indivi dual were also coincident．And it was proved feasibility of DNA fingerprints.
出处
《动物医学进展》
CSCD
2002年第1期46-48,共3页
Progress In Veterinary Medicine
基金
总后卫生部基金课题资助(98M116)
