摘要
目的动态比较研究肺炎克雷伯菌(KPN)所产各种β-内酰胺酶(β-lase)分布情况. 方法采用多底物改良相邻纸片法检测230株,S1期(2000年1月～2001年6月)70株、S2期(2001年7月～2002年7月)70株和S3期(2003年1月～2004年11月)90株;KPN所产各种β-lase. 结果 S1期Kpn总β-lase检出率为28.7%,其中产青霉素酶、头孢菌素酶各1株(1.4%),产广谱酶4株(5.7%),产ESBLs14株(20.0%);S2期70株KPN总β-lase检出率为85.7%,其中产青霉素酶4株(5.7%),头孢菌素酶3株(4.3%),产广谱酶11株(15.7%),产ESBLs 42株(60.0%);S3期KPN总β-lase检出率为64.0%,其中产青霉素酶9株(10.0%),AmpCs酶3株(3.3%),广谱酶3株(3.3%),单纯产ESBLs20株(22.2%),同时产ESBLs+AmpCs产酶株15株(16.7%),酶抑制剂耐药的β-内酰胺酶14株(15.6%,1株IRT、13株IRESBLs),ESBLs总检出率为53.3%;3期均未检出碳青酶烯酶. 结论本组KPN所产各种β-lase的总产酶率、β-lase类型数均随时间而提高.
OBJECTIVE To study the classification and distribution of the β-lactamases produced by Klebsiella pneumoniae in recent years. METHODS To detect the β-lactamases produced by 230 strains of K. pneumoniae in recent years （S1 ,S2 and S3） with a multi-disk synergy test. RESULTS In the S1 , the total production rate was 28.7 %, each strain produced penicillinases or cephalosporinases, 4 strains produced broad-spectrum β-lactamases （BSBLs, 5.7%）; 14 strains produced extended-spectrum β-lactamases （ESBLs, 20.0%）. The total production rate of 70 strains in the S2 was 85.7 %, 4 strains produced penicillinases, 3 strains produced cephalosporinases; 11 strains produced BSBLs （15.7%） , and 42 strains produced ESBLs （60.0%）. In the S3, the total production rate was 64. 0%, 9 strains produced penicillinases （10. 0%）, 3 strains produced AmpC β-lactamases （3. 3%）, 3 strains produced BSBLS （3. 3%）; 20 strains produced only ESBLs （22. 2%）, 15 strains produced ESBLs＋ AmpC β-lactamases （16. 7%）, 14 strains produced inhibitor-resistant β-lactamases （15. 6%, 1 IRTs, 13 IRESBLs）, and 48 strains produced ESBLs （53.3%）. No carbopenem-hydrolyzing β-lactamases were seen in the 3 terms. CONCLUSIONS The total production rate and type of β-lactamases produced by K. pneumoniae in recent years （S1 , S2 and Ss） are raising as the time going on.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2005年第9期1061-1063,共3页
Chinese Journal of Nosocomiology
