摘要
根据国外已发表的鹅细小病毒 (GPV) A株基因组核苷酸序列设计了一对引物 ,对国内两株鹅细小病毒毒株 (GPV CC株 )和 (GPV FS株 )主要结构蛋白 (VP2 - VP3)基因进行 PCR扩增 ,并克隆到p CR3.1T载体 ,分别获得正向、反向连接重组质粒 p VPC1、p VPC2和 p VPF1、p VPF2 ,经酶切鉴定筛选出阳性克隆质粒并进行核苷酸序列分析比较 ,序列分析结果表明 :GPV CC株和 GPV FS株 VP2 - VP3的核苷酸大小分别为 176 4bp和 176 3 bp,其中 GPV CC株与 A株同源性达到 98.9% ;GPV FS株缺失一个碱基 ,与 A株同源性为 93.5 % ,与 GPV CC株同源性为 93%。
The VP2 VP3 genes of two internal isolation stains of Goose Parvovirus(GPV CC strain and GPV FS strain) were amplified by PCR and sequenced after being directly inserted in plasmid PCR3.1 T vector.The sequence analysis showed that the nucleotide sequences of VP2 VP3 genes of two strains were 1764bp and 1763bp.The VP2 VP3 gene of GPV FS strain deleted 1 base.There were 98.9% homology of GPV CC strain and GPV A strain ,indicating that they are closely related.The VP2 VP3 geng of GPV CC strain and GPV FS strain shara 93.5% nucleotide sequence identity and 93% homology of GPV FS strain and GPV A strain.
出处
《动物科学与动物医学》
2001年第3期31-34,共4页
Animal Science & Veterinary Medicine
基金
国家自然科学基金资助项目! (39870 5 5 7)
教育部和总后勤部回国人员启动基金资助项目 !(98H0 2 6 )
