摘要
参考GenBank中的MDPVFM株和GPVB株全基因序列 ,设计并合成一对引物 ,分别对MDPVGD株和GPVGD株的结构蛋白基因 (VP1)进行PCR扩增 ,并克隆到pMD18_T载体 ,筛选到重组质粒并测序。通过对MDPVGD株和GPVGD株的VP1基因的核苷酸序列分析 ,这两种病毒VP1基因大小均为 2 199bp ,核苷酸序列同源性为 87% ,而位于VP1基因上的VP2至VP3基因起始密码子之间的核苷酸序列的差异较大 ,同源性仅为 6 4 %。另外对MDPVGD株和GPVGD株与各地方毒株的VP1和VP3基因核苷酸序列的同源性比较 。
A pair of primers were designed to amplify the VP1 genes of MDPV GD strain and GPV GD strain,according to the sequences of MDPV FM strain and GPV B strain in Genbank,and the genes of interests were obtained by PCR and sequenced after being directly cloned into the pMD18-T.Sequences analysis demonstrated that the VP1 gene of the two strains consisted of 2 199 bp,shared (87 %) homology at the DNA level and the most variable region of the two viruses' capsid polypeptides was located between the start codons of VP2 and VP3,also,compared VP1 and VP3 gene of GD strain with the sequences of other strains deposited in GenBank,indicating that there were little difference among them.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2004年第4期245-247,251,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
广东省农业攻关资助项目 (C2 0 710 )
