摘要
本文以低温脱脂大豆粕为原料 ,比较了多种溶剂对大豆低聚糖的浸提效果 ,确定较优的浸取条件为 :1 %Na2 CO3,温度 55℃ ,时间 2h .经碱溶酸沉后的乳清液 ,采用超滤法除残留蛋白 ,通过正交实验确定的纯化条件为 :温度 2 5℃ ,pH值 6 3,膜压力 2 0psi.离子交换脱盐后 。
Extraction effect of soybean oligosacchride in various solvents was compared using cooled defatted soybean meals as raw material.The better conditions were:1%Na 2CO 3,extraction temperature 55℃ and extraction time 2 hours.After being dissolved by alkali then being precipitated by acid,remaining protein was removed by Ultrafiltration(UF) and through orthogonal tests.The optimum condition was:25℃,pH6.3,and membrane prssure 20 psi.The product was obtained by concentrated under vacuum after desalting by ion exchange.
关键词
大豆低聚糖
低温脱脂
大豆粕
浸提
超滤
纯化工艺
甜味剂
soybean oligosacchride
cooled defatted soybean meals
extraction
Ultrafiltration(UF)
