摘要
真菌诱导子处理人参悬浮培养细胞后,人参皂甙的合成有明显增加,诱导处理改变人参皂甙的积累时程,促进人参细胞培养物中次生产物的外泌,同时增强细胞对蔗糖的摄取、吸收并引起细胞H^+流的变化。
Adding fungal elicitors to the Panax ginseng cell suspension cultures, the biosynthesis of saponin was obviously induced, the total productivity of saponin in cultures could increase more than 30% of the control. During elicitation, the accumulation patterns of saponin in suspension cultured cells were changed, the culture time for maximum biosynthesis of saponin was shortened
2-4 days comparing with that of the control, and about 80% of biosynthetic saponin in elicited cells was secreted into medium, meanwhile the uptake for sucrose in medium of cells was enhanced, and the disturbing of pH in medium was observed, which predicated that an ion exchange occurred between elicited cells and medium.
出处
《实验生物学报》
CSCD
1999年第2期169-174,共6页
Acta Biologiae Experimentalis Sinica
关键词
真菌诱导子
人参
人参皂甙
细胞悬浮培养
Panax ginseng. Fungal elicitor. Cell suspension culture. Saponin.
