摘要
采用幼胚愈伤组织培养技术,观察了亲本和杂种后代及赤霉病粗毒素处理与否的愈伤组织发育过程。结果表明:(1)不同基因型在出愈率、出愈势、愈伤块大小及方差.全能性愈伤组织的比率、褐变率、淘汰率、分化率上均有差异.F_1表现出杂种优势,或优于好亲值,或超出双亲平均值。(2)赤霉素粗毒素处理后,愈伤块受抑制、褐变增加、出苗率降低。在脱离毒素环境后的恢复力和再生能力上,F_1优于亲本,有较强的耐毒能力。(3)选用杂种及改良品种作诱变材料可以提供较大的细胞水平上的选择性。
In this experiment immature embryo callus culture technique was improved and used, and the observation of parents and its hybrid with scab crude toxic processing and nonprocessing was made to study the growth and development of the callus.The results showed., (1) In different genotypes, the potency and the frequency of callus formation, callus sizes and variance, the totipotent callus percentage, brown callus percentage,discarded callus percentage, the regeneration percentage were completely different. F_1 hybrid was prior to to the better parent and excel at parent means, which heterosis.(2)processed by scab crude toxic fluid, the callus growed inhibited, number of brown calli increase and regeneration percentage decrease.For recovery and regeneration after callus leaved toxic circumstance, F_1 hybrid was prior to both parents and show stronger toxic tolerant ability. (3)selected hybrids and improved varieties as mutant materials would more possibly result in successful somatic selection at cell level.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
1991年第1期9-15,共7页
Journal of Huazhong Agricultural University
基金
农业部农02-02课题资助项目
关键词
小麦
赤霉病
幼胚愈伤培养
体细胞
Wheat ( Triticum aestivum L. )
scab
immature embryo callus culture
totipotent callus
somatic selection
