摘要
应用反转录 聚合酶链反应 (RT PCR)技术 ,从伴刀豆球蛋白A(ConA)刺激培养的北京鸭脾淋巴细胞提取的总RNA中扩增得到北京鸭Ⅱ型干扰素 (IFN γ)基因 (DuIFN γ2 )。序列分析表明 ,DuIFN γ2基因长度与报道的鸭IFN γ基因(AF0 87134)长度一致 ,为 497个核苷酸 ,共编码 14 5个氨基酸的成熟蛋白 ,推测其分子量约 17ku。两者核苷酸水平的同源性为 99.6 % ,有 2个位置发生非同义变异 ,其氨基酸序列的同源性为 98.6 % ;与其他禽类的同源性为 6 7.0 %— 6 8.0 % ,与人的同源性为 34.4%。
Interferon γ2 gene (DuIFN γ2) was cloned from total RNA derived from ConA stimulating cultured spleen T Cells of Peking duck by a reverse transcription polymerase chain reaction (RT PCR). Sequencing the gene showed that the length of the DuTFN γ2 gene was identical with the published duck IFN γ gene (AF087134) with 497 nucleotide acids which can code a mature protein with 145 amino acids, speculating its molecular weight 17 ku. The homology of the nucleotide acids of the both genes was 99.6% and the identity of their amino acids was 98.6% iwubg to 2 sites nutation but the identity of the DuIFN γ2 gene and other birds and human was 67.0%—68.0% and 34.4%, respectively.
出处
《中国兽医科技》
CSCD
北大核心
2001年第3期7-10,共4页
Chinese Journal of Veterinary Science and Technology
基金
农业生物技术国家实验室开放课题! (991 0 0 4 - 3)
