摘要
应用RT-PCR技术,直接从成年麻鸭脾淋巴细胞提取的总RNA中扩增出麻鸭γ-干扰素基因(duIFN-γ),并克隆、测序。测序结果表明,麻鸭IFN-γ全序列大小为515 bp,包含一个完整阅读框(495 bp),编码164个氨基酸残基,推导的氨基酸有3个潜在的N-糖基化位点,有5个与二硫键形成有关的半胱氨酸。麻鸭IFN-γ基因序列与AF087134、AF100929、AJ012254的序列完全一致,而与北京鸭(AY166850)核苷酸同源性为99.6%,有2个碱基差异,为非同义突变,氨基酸同源性为98.8%。麻鸭IFN-γ基因与鸡、火鸡和鹌鹑IFN-γ基因核苷酸序列同源性分别为77.2%、78.0%、78.8%,氨基酸序列同源性均67.1%,而与人、狒狒、猪、牛、绵羊、犬、猫IFN-γ核苷酸序列同源性为39.7%～42.1%,氨基酸序列同源性在28.0%～31.7%之间。
Duck IFN -γ gene was amplified from total RNA extracted directionally from ma duck splenocyte by reverse transcription- polymerase chain reaction（RTPCR）, then was cloned and sequenced. The result revealed that the full length sequence of duck IFN -γ gene was consisted of 515 bp, which included one open- reading frame（495 bp）, encoding 164 amino acid residues, there were three potential N -glycosalation sites and five cysteines in deduced amino acid sequence. When the nucleotide sequence of ma duck IFN -γ gene was compared with that of duck IFN-γ genes published previously in GenBank , the sequence was just the same as that of the duck IFN-γ genes （AF087134, AF100929 and AJ012254）, the nucleotide sequence of duck IFN -γ shared 99.6% homology with Peking duck IFN -γ（AY166850）. Homology of the nucleotide sequence of duck IFN-γ with chicken, turkey, quail were 77.2% ,78.0% ,78.8%, respectively, 39.7 % - 42.1% with human, baboon, cat, dog, cattle, pig, sheep, 28.0% - 31.7 % at amino acid level. This study paved the way for future study of biological function and application of duck IFN -γ.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2007年第3期413-417,共5页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家"十五"食品安全重大攻关项目(2001BA804A30-11)
