摘要
采用 70％的乙醇浸提葛根粉末经微波辐射间歇处理 2min，可完成葛根总黄酮的提取，然后采用反向 HPLC法测定了 6个不同种类葛根和葛藤中葛根素、大豆甙的含量。葛根素检测线性范围为 0.115～ 1.38μ g/ml， r=0.9991，平均回收率为 103.31％，变异系数为 1.37％ ;大豆甙检测线性范围为 0.12～ 1.44μ g/ml， r=0.9998，平均回收率为 98.73％，变异系数为 2.43％。实验证明本方法快速、简便、重现性好。
A simple, rapid and accurate quantitative method was established for the determination of puerarin and daidzin in Pueraria Lobata (Wild) by high performance liquid chromatography. The total isoflavone was extracted in P.lobata with 70％ EtOH solution by microwave oven for 2min. Separation was obtained by using Symmetry C 18 column .The retention time for puerarin and daidzin was 4.38min. and 8.15min respectively.The calibration curve was linear in the range of 0.115～ 1.38μ g/ml with r=0.9991 for puerarin and 0.12～ 1.44μ g/ml with r=0.9998 for daidzin. The average recovery rate was 103.31％ with CV=1.37％ for puerarin and 98. 73％ with CV=2. 43％ for daindzin.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2001年第4期64-66,共3页
Food Science
关键词
葛根
葛根素
大豆甙
HPLC法
检测分析
药材
Pueraria Lobata (Wild) Ohwi Puerarin Daidzin High－ performance liquid chromatography
