摘要
应用特异性抗J亚群禽白血病病毒 (ALV_J)的单克隆抗体JE9建立了免疫荧光检测ALV_J抗原和抗体的方法。结果表明 ,在ALV_JADOL_Hcl感染鸡胚成纤维细胞 (CEF) 2 4小时后 ,可以用 1FA检测出阳性细胞 ,感染 72小时后 ,可以检测出最小病毒感染量为≥ 1.2 5TCID50 /孔。对人工感染ALV_J鸡肾脏冰冻切片检测结果证明 ,免疫荧光法可以检测出组织中的ALV_J抗原 ,表现强阳性反应。用重组病毒rBac4817_env感染的Sf9细胞作抗原 ,可以检测出鸡血清中抗ALV_J的特异性抗体。该方法在ALV_J的控制中必将产生重要作用。
A procedure for detecting avian leukosis virus Subgroup J(ALV_J)by immunofluorescence assay(IFA)was developed.The results showed that CEF infected with ALV_J ADOL_Hcl for 24hr was positive to Monoclonal antibody JE9 which were specific to envelope protein of ALV_J in IFA.The sensitivity of detection was not less than 1.25 TCID 50 per well CEF cells in 96_well plate.Frozen section of kideny infected with ALV_J showed positive but SPF chicken tissue negative in IFA.Sf9 cells infected with recombinant baculovirus rBac4817_env could be used to detect antibodies of ALV_J in chickens.The procedure will be very useful in control of ALV_J in the future.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2001年第3期214-216,共3页
Chinese Journal of Preventive Veterinary Medicine
基金
江苏省教育厅自然基金重点项目!(代号OOKJB2 30 0 0 2 )
教育部基金
扬州大学博士启动基金
关键词
禽白血病病毒
J亚群
单克隆抗体
免疫荧光试验
Avian leukosis virus
Subgroups J
Monoclonal antibody
Immunofluorescence assay
