摘要
2008年9月,山东省菏泽市某柴鸡养殖专业户6 000只鸡80日龄时开始发病,至120日龄时死亡率高达15%。病鸡颜面苍白,机体进行性消瘦,瘫痪,最后衰竭死亡。部分鸡只可见单侧肢体麻痹症状和皮肤型肿瘤。对66份采自发病鸡群的血清及新生羽毛囊进行血清学检测。琼脂扩散试验结果显示所采的66份待检羽髓抗原MDV阳性率为59.09%,血清中MDV抗体阳性率为65.16%,抗原、抗体双阳性率为37.88%。采用酶联免疫吸附试验(ELISA)检测鸡血清中ALV-J、REV抗体,结果显示ALV-J抗体阳性率为7.58%,REV抗体检测全部呈阴性。剖检病鸡可见内脏器官普遍肿大,肝、脾、肾表面及切面有大小不等的灰白色肿瘤样结节。病理组织学观察肿大的器官和组织内都有不同程度的淋巴细胞增生,并可在肝、脾、肾、腺胃等组织内同时观察到淋巴细胞样瘤细胞和髓样瘤细胞2种典型的肿瘤灶。免疫组织化学检测结果表明,发病鸡组织内同时存在MDV和ALV-J抗原的阳性信号。PCR检测结果显示6只来检病鸡MDV和ALV-J均呈阳性,都扩增出相应的条带,REV未扩增出任何条带,PCR呈阴性。以上结果表明地方柴鸡鸡群中存在MDV和ALV-J的共感染,提醒我们应当注意地方柴鸡肿瘤性疾病的预防和净化工作。
An outbreak of tumors diseases occurred in Shandong free range chickens on a commercial farm of Shandong Province in China during September 2008. The flock containing 6 000 chickens, and the symptoms were first seen at 80-day-old. The mortality reached 15% at 120- day-old. The main symptoms were characterized by emaciation, ruffled feathers, paralysis and visceral tumors. Some birds showed unilateral limb paralysis and skin tumors. 66 serum and feather samples were collected from the sick chickens to evaluate the presence of antibodies to MDV, ALV-J and REV using agar diffusion reaction and enzyme-linked immunosorbent assay （ELISA） technique. The results of agar diffusion reaction showed that the MDV antigen positive rate was 59.09%, and MDV antibodies positive rate was 65.16%, antigen-antibody-positive rate was 37.88%. The overall seroprevalence of ALV-J antibodies was 7.58%, while the antibodies to REV were negative. Numerous similar nodular lesions and resembling tumors in one or more tissues were seen at necropsy. Some organs showed intumescence. Histopathological findings revealed large islands of leukomonocytes or myelocytes in tissues containing tumors. Immunostaining for MDV, ALV-J and REV monoclonal antibody revealed a diffuse presence of MDV and ALV-J antigen. PCR analysis revealed the presence of MDV and ALV-J proviral sequences in DNA of affected tissues. All the findings observed in this case were considered to be related with the MDV and ALV-J mixed infection. Prevention and decontamination to these diseases in local free range chickens should be noticed.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2009年第8期1215-1221,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
山东省科技公关项目资助(2006GG2209010)
关键词
地方柴鸡
J亚群白血病
马立克氏病
血清学
免疫组织化学
PCR
local free range chickens
subgroup J of avian leukosis
Marek＇s disease
serology
immunohistochemistry
PCR
