摘要
由致病性Ⅰ型马立克氏病病毒(MDV)特异性的单克隆抗体H_(19)制备亲和层析柱,从感染重组杆状病毒BP38Ⅱ的昆虫细胞系Sf9细胞中提纯MDV的pp38基因表达产物,以此免疫小鼠制备抗血清,测定其与不同型MDV毒株感染的鸡胚成纤维细胞免疫反应性,同时也用不同型MDV毒株(HVT,Z4)制备的抗血清和自然感染发病鸡血清分别与感染重组病毒的Sf9细胞进行免疫交叉反应.试验结果表明,完整的Ⅰ型MDV来源的pp38基因产物与所试Ⅱ型Z4株和Ⅲ型HVT Fc126毒株在抗原性上有显著交叉反应.这一结果表明,在Ⅱ型和Ⅲ型MDV毒株中可能存在着pp38的类似物.
Marek's disease virus (MDV) 38kD phosphorylated protein (pp38),which was identified by monoclonal antibody (mAb) H19 specific to pathogenic serotype Ⅰ of MDV, has been recognized as a transformation-associated antigen specific to serotype Ⅰ of MDV. The recombinant pp38 was prepared and purified from pp38-expressing recombinant baculovirus infected Sf9 cell lysates through a mAb H19- affinity column. In indirect fluorescence antibody test, the mouse sera against the purified recombinant pp38 not only gave a high titer to serotype Ⅰ strain GA- infected CEF but also gave a reasonable liters to serotype Ⅱ strain Z4- and serotype Ⅲ HVT strain Fcl26-infected CEF. In another way, the pp38- expressing recombinant baculovirus infected Sf9 cells reacted with anti-serotype ⅠMDV chicken sera as well as with anti-serotype Ⅱ and serotype Ⅲ MDV chicken sera respectively. The results imply that both serotypes Ⅱ and Ⅲof MDV probably contain a homologue to pp38 of serotype Ⅰ .
出处
《微生物学报》
CAS
CSCD
北大核心
1994年第5期393-397,共5页
Acta Microbiologica Sinica
基金
国家自然科学基金
江苏省教委基金资助项目
关键词
马立克氏病
病毒
磷蛋白
交叉免疫
Marek's disease virus, Recombinant 38kD phosphorylated protein
