摘要
目的：建立同时测定绿原酸和头孢唑啉血浆浓度的高效液相色谱法。方法：色谱柱：Ｓｐｈｅｒｉｓｏｒｂ Ｃ１８；流动相：甲醇－０．１ｍｏｌ／Ｌ磷酸二氢钾（３０：７０，ｖ／ｖ），磷酸调节 ｐＨ值至３；流速：１．２ｍｌ／ｍｉｎ；检测波长：２７５ｎｍ（绿原酸）和３２６ｎｍ头孢唑啉）。测定大鼠静脉给药的血浆浓度。结果：药物浓度与峰高线性关系良好（ｒ＝０．９９７和０．９９８）；绿原酸日内和日间ＲＳＩ）均小于４％，头孢唑啉日内和日间ＲＳＩ）均小于６％；两种药物高、中、低浓度平均加样回收率分别为９３．３０％、９３．３５％、９６．４０％和９３．４１％、９６．６０％、９２．３６％。与绿原酸合用降低体内头孢唑啉浓度。结论：该法适于绿原酸和头孢唑啉血浆浓度的同时监测。
OBJECTIVE:To establish a HPLC method for simultaneous determination of chlorogenic acid and cefazolin in rat plasma.METHODS: Chromatographic determination was performed on spherisorb Cis column with methyl alcohol - 0. 1mol/L potassium ethydrogen phosphate(30 : 70v/v,pH 3.0)as the mobile phase and a flow rate of 1.2ml/min .Detection wavelengthes were UV 275nm for chlorogenic acid and 326nm for cefazolin .The concentrations of chlorogenic acid and cefazolin in rat plasma were detected .RESULTS: The calibration curves were linear with the correlation coefficients of 0.997 and 0.998, repective-ly.The RSDs for the between ?day and within ?day were both lower than 4% for chlorogenic acid and 6% for cefazolin, re-spective.The mean recoveries were 93.30% , 93.35% and 96.40% for chlorogenic acid, and 93.41% , 96.60% and 92.36% for cefazolin at large, middle and low dosages, respectively .The concentrations of cefazolin in rat plasma were decreased in co-administration with chlorogenic acid .CONCLUSION: The study provides a simple method for simultaneous determination of the plasma levels of chlorogenic acid and cefazolin.
出处
《中国药房》
CAS
CSCD
2001年第4期208-209,共2页
China Pharmacy
