摘要
目的:建立大鼠原位肠-肝灌流模型,运用该模型研究绿原酸在大鼠肠、肝中的代谢转化。方法:采用液相色谱质谱联用(HPLC-MS)法测定大鼠原位肠-肝灌流模型灌流液中绿原酸及其代谢产物咖啡酸、阿魏酸和马尿酸。结果:绿原酸十二指肠给药后,灌流液中主要活性代谢产物为阿魏酸,同时在灌流液中也检测到少量活性代谢产物咖啡酸和大量代谢终产物马尿酸。结论:大鼠原位肠-肝灌流模型适用于绿原酸生物转化和代谢动力学的研究;绿原酸在肠中存在广泛的代谢。
AIM： To establish a model of in situ perfused rat intestine-liver preparation and HPLC-MS method in the deten＇nination of chlorogenic acid, caffeic acid, hippuric acid and ferulic acid in gate chlorogenic acid metabolism perfusate, and investiin intestine and liver of rats by the model. METHODS： Chlorogenic acid and its metabolites （caffeic acid, ferulic acid and hippuric acid） were detected by HPLC-MS from samples of in situ perfused rat intestine-liver model. RESULTS： Main active metabolite of chlorogenic acid is ferulic acid in in situ perfused rat intestine-liver model after intraduodenal injection to rats, and trace caffeic acid and abundant hippuric acid were also found in perfusate. CONCLUSION： In situ perfused rat intestine-liver models are suitable for the study of metabolism of chlorogenic acid in intestine and liver of rats. Chlorogenic acid was extensively metabolized in rat intestine.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2006年第12期1340-1344,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
国家863计划课题(№2003AA2Z347B)
关键词
大鼠原位肠-肝灌流模型
绿原酸
咖啡酸
阿魏酸
马尿酸
代谢
in situ perfused rat intestine-liver model
chlorogenic acid
caffeic acid
ferulic acid
hippuric acid
metabolism
