摘要
目的 探讨NAD(P)H :醌氧化还原酶 (NQO1)基因C6 0 9T位点基因多态性与散发性阿尔茨海默病 (SAD)遗传易感性的关系。 方法 应用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)方法检测了 12 0例SAD患者和 12 2名健康老年人的NQO1基因多态性分布特征。 结果 SAD组C等位基因频率低于对照组 ,SAD组为 45 0 % ,对照组为 5 7 0 % (OR =0 6 3,P <0 0 5 )。SAD组C/C基因型频率明显低于对照组 ,SAD组为 12 5 % ,对照组为 2 9 5 % (OR =0 34 ,P <0 0 1)。 结论 NQO1基因多态性与SAD发病明显关联 ,NQO1基因 6 0 9序列C/C基因型、C等位基因与SAD发病呈明显负关联。NQO1基因 6 0 9序列C等位基因对SAD发病可能有保护作用。
Objective To explore the association between the cDNA polymorphism of NAD(P)H:quinone oxidoreductase(NQO1)at gene locus 609 C→T mutation and the genetic sensitivity of the sporadic Alzheimer's disease(SAD). Methods The polymorphism of NQO1 gene was detected using polymerase chain reaction restriction fragment length polymorphism(PCR RFLP) technique for 120 patients with SAD and 122 normal controls. Results The C/C genotype and C allele frequencies in SAD were 12 5% and 45 0% decreased than in controls. Conclusions Our results indicated that SAD was inversely associated with C/C genotype and C allele of NQO1 gene. C allele of NQO1 gene may protect people from SAD.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2001年第2期93-95,共3页
Chinese Journal of Geriatrics
基金
北京市卫生局老年神经变性疾病临床与基础研究重点学科项目
