摘要
[目的]探讨NQO1基因7个常见位点在江苏汉族人群中的多态性分布。[方法]应用聚合酶链式反应-限制性片断长度多态性(PCR-RFPL)、等位基因特异性引物-聚合酶链反应技术(AS-PCR)及普通Taqman探针等技术方法,对658例正常体检样本NQO1基因多位点进行分析。[结果]NQO1基因相关位点野生型纯合子、杂合子、突变纯合子3种基因型的分布频率分别是:rs1800566 44.8%,36.4%,18.8%;rs4986998 93.5%,6.2%,0.3%;rs10517 16.1%,60.1%,23.8%;编码区rs11555215和非编码区rs1050873,rs1063556,rs80564684个位点未检测到杂合子和突变纯合子。[结论]本研究结果与国内外相关报道有一定的差异。对了解不同种族、不同地域人群NQO1基因的遗传特征具有一定的参考价值。
[ Objective] To investigate the polymorphisms of NQO1 genes in Jiangsu ban population. [ Methods] PCRRFPL, AS-PCR and Taqman probe technique was used to perform genotyping. [Results] The genotyp results of 658 individuals showed that the population distribution frequency of related sites genotypes of wild type, heterozygote, homozygous variant were rs1800566 44.8%, 36.4%, 18.8%; rs4986998 93.5%, 6.2%, 0.3%; rs10517 16.1%, 60.1%, 23.8% respectively. Heterozygote and homozygous variant were not detected in another four sites. [ Conclusion ] These results differ from those of abroad and domestic among other population. It is significant to discuss genetic characteristics of NQO1 in different races and areas.
出处
《现代预防医学》
CAS
北大核心
2007年第5期835-838,共4页
Modern Preventive Medicine
基金
国家自然科学基金资助项目(30571540
30671732)
