摘要
目的 :建立和应用连续酸 -尿素 -聚丙烯酰胺凝胶电泳 (CAU- PAGE)纯化人和兔中性粒细胞防御素组分(HNP- 1～ 3和 RNP- 1～ 5 )。 方法 :从人外周血或兔人工无菌性腹水中分离中性粒细胞 ,用 5 %冰醋酸直接提取 ,经 CAU- PA GE洗脱 ,获取纯化的 HNP- 1～ 3或 RNP- 1～ 5组分。以聚丙烯酰胺凝胶过滤作为参比方法。 结果 :1次洗脱电泳可从 5× 10 8个细胞中获得纯化的 HNP组分约 1.5 0 mg或 RNP组分 2 .5 0 mg,并保持天然的生物活性。 结论 :CAU- PAGE是一种较简便、快速。
Objectives: To develop a preparative method of continuous acid urea polyacrylamide gel electrophoresis (CAU PAGE) to purify the defensin components from human and rabbit neutrophils (HNP 1~3 and RNP 1~5). Methods: Neutrophils were isolated from human peripheral blood or rabbit artificial aseptic ascites. The components of defensin were extracted by 5% acetic acid directly followed by elution of CAU PAGE, and purified HNP 1~3 or RNP 1~5 were harvested. Polyacrylamide gel filtration was used as the method for comparison. Results: For each elution circa 1.50 mg of purified HNP or 2.50 mg of RNP were obtained from 5×10 8 of neutrophils, and their natural biological activity was preserved. Conclusions:CAU PAGE is a relatively simple, rapid, economical method for purifying cationic peptides as the components of defensin.
出处
《医学研究生学报》
CAS
2001年第2期106-109,共4页
Journal of Medical Postgraduates
基金
国家教育部留学回国人员科研基金!资助 (编号 :0 960 65 )
