摘要
采用牛血清白蛋白(BSA)——肽抗原偶联技术,制备了免抗人防御素多克隆抗血清。并以此抗血清用免疫细胞化学染色法检查了一系列正常白细胞和白血病细胞株的免疫细胞化学反应。结果显示正常人中性粒细胞、急性髓性白血病细胞株KG1和HL-60细胞呈现强阳性反应。在正常人单个核白细胞、人红白血病K562细胞株及小鼠骨髓瘤SP2/0细胞株中均为阴性反应,甚至在健康家兔及豚鼠中性粒细胞中亦呈阴性反应。本实验提示防御紊可能作为粒系白细胞分化标志。
Our laboratory isolated three defensin molecules from the circulating neutrophils of healthydonors.The three molecule mixture was conjugated with BSA and immunized New Zealand rab-bit.ELISA showed that the rabbit antisera was of a very high titters,which were specific for hu-man defensins.Immunoperoxidase staining with this rabbit antisera indicated that normal humanneutrophils,acute myeloid leukemia cell lines KG1 and HL-60 were highly positive.In con-trast,normal mononuclear leukocytes,erythrocytes,erythroleukemia cell line K562 were all neg-ative,even rabbit and guinea pig neutrophils had no response to this rabbit anti-human de-fensins antisera.Our preliminary experiments suggested that the rabbit antisera was highly spe-cific for human defensins.Probably,defensin is a differentiation antigen of granunocyte lineage,and antibody against human defensins would be a candidate immunological reagent specific for a-cute myeloid leukemia.Further work for this should be done.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1993年第6期336-338,共3页
Chinese Journal of Immunology
基金
国家自然科学基金资助项目
