摘要
目的研究肝纤维化形成过程中肝窦壁层粘连蛋白、Ⅳ型胶原蛋白的变化。方法运用DMN大鼠肝纤维化模型,通过层粘连蛋白、Ⅳ型胶原蛋白的免疫组化观察,系统研究24wk大鼠肝纤维化形成过程中肝窦壁基底膜成分的动态变化。结果 DMN大鼠肝纤维化形成过程中肝窦壁Ⅳ型胶原蛋白表达造模1wk后减少,以后逐渐增强,停止造模后逐渐消退;肝窦壁层粘蛋白表达逐渐增强,停止造模后逐渐减弱。结论大鼠肝纤维化形成过程中,肝窦壁功能性基底膜被破坏,取而代之由于层粘连蛋白、Ⅳ型胶原蛋白共同沉积,形成高密度基底膜,发生肝窦毛细血管化;停止造模后,肝窦基底膜可自行消退。
AIM To Investigate the variations of LN and Col Ⅳ in periainusoids during the formation of liver fibrosis in rats. METHODS Liver fibrosis was Induced in rats after administration of dimethyinitroasmine (DMN) intraperitoneslly three times a week for 4 weeks. The animals were killed on day 2 and weeks 1, 2, 3, 4, 5, 6, 8, 12 and 24. Immunohistochemistry for LN and Col Ⅳ was used to outline the formation of basement membrane on hepatic sinusoida. PESULYS In the early stage of the experiment, the perisinusoidal Col Ⅳ decreased, and then gradually increased and reached their peaks at the 4th week; the perisinosoidal LN increased gradually. Perisinusoidal Col Ⅳ and LN declined slowly after administration of DMN was stopped. CONCLUSION During the formation of the liver fibrosis in rate, the sinusoidal functional basement membrane is destroyed, resulting in the high density basement membrane, which will be degraded.
出处
《世界华人消化杂志》
CAS
2001年第3期260-262,共3页
World Chinese Journal of Digestology
基金
1998年国家杰出青年科学基金
No.39825128
上海市教委曙光计划资助课题
No.99SG44
关键词
肝硬化
蛋白质类
胶原
肝窦毛细胞血管化
免疫组织化学
病理
liver cirrhosis
proteins
collagen
hepatic sinusoid capillarization
immunohistochemistry
rats
