摘要
以免疫组化方法对CCl_4加低酒精饮水及低胆碱饲料诱发的大鼠早期肝纤维化形成及消退过程中纤维连接蛋白(FN),层粘连蛋白(LN)和Ⅳ型胶原蛋白(Col Ⅳ)的演变作了观察;何时用免疫单扩散法测定了实验各期大鼠血浆FN。发现实验早期在肝细胞坏死区扩张的血窦壁及周围基质中首先见FN增多,形成早期间隔;以后LN和Col Ⅳ也在此处血窦壁及间隔内血管和新生小胆管的基膜处沉积,但基质中无明显沉积。随实验进展,FN继续增多,并先于胶原纤维在肝内形成完全间隔。肝纤维化消退时,FN消退最快,LN和Col Ⅳ则较慢。血浆FN在实验早期明显上升,以后逐步回降,其变化与肝组织中FN的量并不平行。
Sequential immunohistochemical observations were made on fibroneetin (FN), laminin (LN) and collagen type Ⅳ (Ool Ⅳ) from early formation to the subsidence of rat hepatic fibrosis which was induced by carbon tetrachloride, plug drinking water containing low concentration of alcohol and a low choline diet, Plasma FN was determined simultaneously by the single radial diffusion test.We found that FN dramatically increased along the wall of sinusoids which had dilated forming septa after hepatocytic necrosis and in the matrix around it during the initial period of the experiment, Later, LN and Ool Ⅳ deposited on the wall of sinusoids mentioned above and on the basement membrane of blood vessels and newly formed small bile ducts within the septa. However, no noticeable LN and Ool Ⅳ were observed in the matrix there. With progression of the experiment, FN increased continuously forming complete septa before formation of collagen septa. When the liver recovered from fibrosis, FN was the quickest to subside, LN and Ool Ⅳ deposition subsided more slowly.Plasma FN rose markedly during the initial period of the experiment and decreased thereafter, but did not parallel changes of FN in the livers. The rolea and the dynamic changes of FN, LN and Ool Ⅳduring fibrogenesis ware-discussed.
出处
《上海医科大学学报》
CSCD
1993年第5期380-384,T022,共5页
Journal of Fudan University(Medical Science)
基金
国家自然科学基金
关键词
肝纤维化
纤维连接素
蛋白
免疫学
hepatic fibrosis
fibronectin
laminin
collagen type Ⅳ
carbon tetrachloride
immunohistochemistry
