摘要
目的 :对 2 0余种中药材和中成药进行抗肿瘤细胞毒生物活性测定。方法 :采用HPLC法 ,色谱柱KR10 0 5C18,流动相乙腈 水 (4 3 :5 7) ,检测波长 2 10nm ,流速 0 .8mL/min。结果 :回收率分别为 99.8% (RSD 2 .6 % ,n =6 ) ;98.8%(RSD 1.5 % ,n =6 ) ;日内和日间精密度分别为RSD1.4% ,n =6 ;RSD1.8% ,n =4。结论 :中药重楼和云南白药的水、甲醇和乙醇提取物对 6种人体肿瘤细胞均有明显的抑制作用 。
Objective:To establish a HPLC for determination of Gracillin in Rhizoma Paridis and Yunnan white on the bases of determining anti tumor cytotoxic bioactivities of more than 20 varieties of chinese medical materials and traditional Chinese patent medicines.Methods: The determination was carried out by HPLC on KR 100-5C18 column, with acetoitrile water (43∶5) as a mobile phase. The detection wavelength was 210nm. and the flow rate was 0.8ml/min.Results: The recoveries are 99.8% (RSD in 2.6%, n=6) and 98.8% (RSD is 1.5%, n=6), respechrely. The within day and between day accuracies are 1.4% of RSD(n=6) and 1.8% of RSD (n=4), respectively.Conclusion: The results showed that water, methanol and alcohol extracts of Rhizoma Paridis and Yunnan white had strong inhibition on 6 kinds of neoplasm cells of human body and their component Grarillin also had the inhibition on tumor cells.
出处
《中成药》
CAS
CSCD
北大核心
2001年第3期212-215,共4页
Chinese Traditional Patent Medicine
