摘要
从果蝇成虫中抽提总 RNA,RT- PCR扩增编码伏蝇素 diptericin的 c DNA,克隆并测定了全序列 .将该 c DNA亚克隆到融合表达载体 p MAL- CR1上 ,转化大肠杆菌 BL2 1菌株 ,进行了高效的可溶性融合表达 .通过离子交换层析纯化融合蛋白 ,经 Xa因子酶切后得到的重组 diptericin具有抗菌活性 .
A cDNA encoding diptericin was extracted from Drosophila adults through RT RCR.The cDNA was cloned into pGEM T vector.After restriction analysis and DNA sequencing,the gene was ligated with fusion expression vector pMAL CR1 and transfected into E.coli BL21.The positive clone was induced by IPTG.The expression product was purified by ion exchange chromatography and digested by factor Xa.The final product,rDiptericin,exhibited antibacterial activity.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2001年第2期182-184,共3页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家高技术研究发展计划项目! (No.10 1-0 1-0 2 -0 3)&&
