摘要
目的:观察乙型肝炎病毒(HBV)S区DNA疫苗pCR3.1-S诱导BALB/C小鼠(H-2~d)的特异性免疫应答,及其对稳定表达HBsAg的小鼠肥大细胞瘤细胞P815(P815-HBV-S)成瘤性的影响。方法:肌肉注射DNA疫苗;背部皮下接种P815-HBV-S细胞,观察成瘤情况;ELISA法检测小鼠血清抗HBs;4h^(51)Cr释放法检测小鼠脾细胞CTL活性。结果:pCR3.1-S体外可表达HBsAg;小鼠接种该疫苗后血清450nm A值为0.38,强化免疫后达0.87;pCR3.1-S组CTL细胞杀伤活性为51.1％,对照组为20.5％(P<0.01)。接种P815-HBV-S细胞后对照组成瘤率100％,pCR3.1-S小鼠成瘤率为16.7％,对照组小鼠6周后全部死亡;pER3.1-S组6周后存活率为87.5％。结论:HBV S区DNA疫苗具有良好的免疫原性,能够诱导小鼠产生特异性体液免疫和细胞免疫应答,对体内HBV感染具有预防及治疗作用。
To observe the specific immune responses and the protection against P815 mastocytoma cells stable expressing HbsAg in H-2d mice after immunized by DNA vaccine of HBV S gene. Methods: The immunization was performed by intramuscular injection of DNA and subcutaneous injection of P815-HBV-S. Serum anti-HBs was detected by ELISA. HbsAg secific cytotoxic T lymphocyte(CTL)activity was measured by 51 Chromium release assay. Results:The expression of HB-sAg was demonstrated by indirect immunofluorescent method. The 450nm A value of serum in immunized mice was 0.87. HBsAg specific CTL activity was 51.1%. HBV-SDNA vaccine could evidently inhibit the tumor growth,prolong the survival period( >38.2 d),and improve the survival rate. Conclusion: pCR3.1-S DNA vaccine has a strong antigenicity and marked killing effect to HBV infected cells in vivo.
出处
《天津医药》
CAS
北大核心
2001年第4期217-219,共3页
Tianjin Medical Journal
基金
国家自然科学基金
