摘要
目的 观察编码鼠 IL - 12的真核表达载体对 HBVDNA疫苗诱导 BAL B/ c小鼠免疫应答的影响 .方法 肌肉注射 DNA疫苗 ,EL ISA法检测小鼠血清抗 HBs,4h5 1 Cr释放法检测小鼠脾细胞 CTL杀伤活性 .结果 免疫 8wk后 ,单纯注射 p CR3.1- S及共注射 IL - 12真核表达载体的小鼠血清 45 0 nm A值分别为 0 .87± 0 .1及 1.6 7± 0 .15 .CTL 细胞杀伤活性分别为 (5 0 .5± 6 .4) %及 (73.3± 8.8) % ,两组均有明显差异 (P<0 .0 1) ,脾细胞悬液经抗 CD4+ 单克隆抗体处理后 CTL 细胞杀伤活性分别为 (48.3± 5 .9) % ,(75 . 6±9.1) % ,抗 CD8+单克隆抗体处理后分别为 (10 .6± 1.4) % ,(16 .9± 2 .3) % .结论 IL - 12的真核表达载体能够提高小鼠对 DNA疫苗的免疫应答 ,CTL细胞杀伤活性主要由 CD8+执行 .基因疫苗可能用于预防及治疗
AIM To observe the immune responses to gene vaccines coding HBV surface antigen and interleukin 12 in BALB/c (H-2d) mice. METHODS The immunization was performed by intramuscular injection in this experiment. Anti-HBs in serum was detected by ELISA. HBsAg specific cytotoxic T lymphocytes (CTLs) activity was measured by 51 Chromiun release assay. RESULTS Eight weeks after immunization, the serum of mice A value in 450 nm codelivried IL-12 vaccine (1.67±0.15) was significantly higher than that of mice injected HBV-S DNA vaccine (0.87±0.1). CTLs activity of the mice injected HBV-S DNA vaccine and codelivried IL-12 vaccine were (50.5±6.4)% and (73.3± 8.8 )% respectively. After adding anti-CD4 + monoclonal antibody in spleen cells, CTLs activity of the mice injected HBV-S DNA vaccine and codelivried IL-12 vaccine was (48.3± 5.9 )% and (75.6±9.1)% respectively. CTLs activity was (10.6±1.4)% and (16.9±2.3)% respectively after adding anti-CD8 + monoclonal antibody in spleen cells. CONCLUSION The results showed that the DNA vaccine of pCR3.1-S had strong antigenecity in cellular and humoral immunity which can be promoted by vector coding murine IL-12. CTLs activity was performed by CD8+ cells. DNA vaccine against HBV may be useful for both prophylactic and therapeutic purposes.
出处
《第四军医大学学报》
2000年第7期805-807,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金!资助项目 ( 3 9770 665 )
