摘要
建立了一个快速、简单、准确的固相萃取和高效液相色谱相结合的测定苦瓜甙A含量的方法。样品经石墨碳固相萃取管 (3mL/ 2 5 0mg)纯化后以高效液相色谱检测。色谱柱为C18,流动相为V(乙腈 )∶V(甲醇 )∶V(5 0mmol/L磷酸二氢钾缓冲液 ) =2 5∶2 0∶6 0 ,流速为 0 .8mL/min ,检测波长为 2 0 8nm。标准曲线自 10mg/L到 10 0 0mg/L呈线形关系 (r2 =0 .9992 )。该方法具有很好的重现性 ,日内或日间的相对标准偏差和相对平均误差均小于 10 %。样品回收率大于 90 %。
A rapid, simple and accurate method for the determination of momordicoside A has been established using solid phase extraction (SPE) followed by high performance liquid chromatography (HPLC). Sample was processed by SPE on a Carb cartridge (3 mL/250 mg), and was then determined by HPLC on a C 18 column (4.6 mm i.d.×250 mm, 5 μm) with V (acetonitrile)∶ V (methanol)∶ V (50 mmol/L potassium dihydrogen phosphate buffer)=25∶20∶60 as mobile phase (0.8 mL/min) and UV detection at 208 nm. The calibration curves were linear from 10 mg/L to 1?000 mg/L ( r 2 = 0.999 2 ). The analytical method was shown to be highly reproducible, giving all of the relative standard deviations and relative mean errors less than 10% for both intra day and inter day determinations. The absolute recoveries were greater than 90%.
出处
《色谱》
CAS
CSCD
北大核心
2001年第2期128-131,共4页
Chinese Journal of Chromatography
基金
ThisworkwassupportedbythekeyresearchprogramofEducationMinistryofChina
关键词
高效液相色谱
固相萃取
苦瓜甙A
苦瓜
测定方法
high performance liquid chromatography
solid phase extraction
momordicoside A
bitter melon
