摘要
采用反相高效液相色谱法 ,一次进样、同时测定食品中的人工合成甜味剂 (糖精钠、安赛蜜、甜味素 )、防腐剂(苯甲酸、山梨酸 )、咖啡因、可可碱和茶碱。以AlltechEconosphereC18柱 (15 0mm× 4 6mmi.d .,3μm)为分离柱 ,10mmol/LNaH2 PO4 (pH 4 0 0 ) 乙腈 (体积比为 90∶10 )为流动相 ,采用二极管阵列检测器进行检测。整个分离过程在 2 3min内完成。样品平均加标回收率为 78 5 %～ 10 7 2 %。
A novel method is proposed for the simultaneous separation and determination of eight food additives, acesulfame potassium (AK), aspartame (ASP), benzoic acid (BA), caffeine (CA), saccharin sodium (SA), sorbic acid (SOR), theobromine (TB) and theophylline (TP) by reversed phase high performance liquid chromatography. The separation was achieved within 23 min by using an Alltech Econosphere C 18 column with 10 mmol/L NaH 2PO 4 (pH 4.00) acetonitrile (90∶10, V/V ) as mobile phase. The qualification and quantitation were accomplished by using a photodiode array detector. The detection limits ( S/N =3) for all analytes were below mg/L level. Under the experimental conditions, other common food additives and organic acids such as cyclamate, citric acid, malic acid, tartaric acid and ascorbic acid, did not interfere with the determination. The method has been successfully applied to the analysis of various foods as well as pharmaceutical preparation, and the average recoveries for real samples ranged from 78.5% to 107.2%.
出处
《色谱》
CAS
CSCD
北大核心
2001年第2期105-108,共4页
Chinese Journal of Chromatography
基金
国家出入境检验检疫局科研基金资助项目! (K0 3 1 1999)
