摘要
目的构建疱疹病毒 gD2基因多聚核苷酸疫苗并在实验动物中得到表达。方法将构建的 pcDNA3-gD2的质粒 DNA注射入豚鼠的舌肌中，每周 1次，共 3次，最后 1次免疫 48 h后引颈处死豚鼠，并取其舌、肝、脾、肾、心脏及股四头肌，用免疫组化法 (SP法 )检测 gD2抗原的表达。结果重组质粒组 gD2真核表达载体免疫接种后，均可在舌肌测到抗原表达，而对照组均为阴性。结论重组质粒 gD2真核表达载体可以在豚鼠舌肌中表达，对建立基因免疫防治疱疹病毒感染奠定一定基础。
Objective To construct Herpes simplex virus-2 glycoprotein D polynucleotide vaccine, and to detect the glycoprotein D2(gD2) expression in guinea pig′ s tongue by intramuscular injection of gD2 polynucleotide vaccine. Methods The recombinant plasmids HSV-gD2 polynucleotide vaccine was constructed by molecular cloning technique. The guniea pigs were intramuscularly injected with 0.75％ bupivacaine, after 7 days, they were divided into 3 groups(4 guinea pigs per group) and intramuscularly injected with 100μ L 0.09％ NaCl、 pcDNA3、 gD2-pcDNA3, three times at 7 day intervals respectively. All guinea pigs were sacrificed 2 days after the last injection, and the tongue, liver, kidney, spleen and quadricep muscle were removed for immunohistochemical assay (SP method). Results The recombinant pcDNA3-gD2 plasmid was constructed succuessfully. Immunohistochemical staining for gD2 was positive in the tongue injected with pcDNA3-gD2, but negative in the liver, kidney, spleen, heart and quadricep muscle. Conclusion Herpes simplex virus-2 glycoprotein D polynucleotide vaccine was constructed successfully, and gD2 antigen can be expressed effectively in the tongue after injection of the HSV-gD2 polynucleotide vaccine in it.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2001年第1期53-54,共2页
Chinese Journal of Dermatology
基金
广东省科委自然科学基金资助! (960671)
