摘要
依据国外已发表的疱疹病毒（ＨＳＶ）糖蛋白Ｄ基因（ｇＤ基因）的核苷酸保守区序列设计了一对引物，建立了用疱疹病毒ｇＤ基因做疱疹病毒诊断基因的ＰＣＲ方法。对４８例拟诊为疱疹病毒感染的临床标本进行病毒细胞培养和ＰＣＲ检测。结果表明，ＰＣＲ对ＨＳＶ２感染的敏感性为８４％（２０／２４），特异性为９１％（２２／２４）。经标准株及限制性内切酶的酶切证实和序列分析，表明该方法特异。
Polymerase chain reaction (PCR) employing specific primers corresponding to conserved sequence of gD gene of HSV 2 strain was applied to 48 clinical specimens of patients with genital herpes or those who had a history of genital herpes. Our results showed that the sensitivity and specificity of PCR for HSV 2 in fection were 84% (20/24) and 91% (22/24) respectively. The amplified productsor HSV 2 standard strain identified by sequence analysis and restriction endonuclease analysis showed that the sensitivity and specificity of PCR were very high.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
1998年第5期292-294,共3页
Journal of Clinical Dermatology
基金
广东省科委自然基金
