摘要
目的 研究 5 -氟尿嘧啶 (5 -FU)术前化疗诱导胃癌细胞凋亡的作用及其与癌细胞Ki- 6 7抗原、增殖细胞核抗原 (PCNA)表达的关系 ,以评价 5 -FU术前化疗的价值。方法 对 2 8份胃癌手术标本 (分为术前化疗组和未化疗对照组 ,n=14)应用 3′-OH末端标记法 (TUNEL)检测胃癌手术标本的凋亡细胞 ,电镜观察凋亡细胞的超微结构 ,免疫组织化学法检测癌细胞Ki- 6 7、PCNA表达。结果 5 -FU术前化疗组手术标本癌细胞凋亡率较未化疗组明显提高 (P <0 .0 1) ,而Ki- 6 7、PCNA抗原表达则明显低于未化疗组 (P <0 .0 1)。结论 5 -FU通过诱导胃癌细胞凋亡杀伤癌细胞 ,尤其是具有侵袭、增殖潜能的癌细胞。提示 5
Objective By investigating the effect of apoptosis of the gastric carcinoma cells induced by pre-operative treatment with 5-fluorouracil (5-FU) and its relationship to the expression of Ki-67 and proliferating cell nuclear antigen (PCNA) of carcinoma cells to evaluate the value of pre-operative chemotherapy with 5-FU.?Methods The 28 surgical specimens of gastric carcinoma from two groups (pretreated and non-pretreated control, n=14 each) were examined for apoptosis by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick end labeling (TUNEL) method and by electron microscopy.The expression of Ki-67 and PCNA of carcinoma cells was determined by immunohistochemistry.?Results The apoptosis index of cells in pre-treated group was significantly higher (P<0.01), and the expression of Ki-67 and PCNA of carcinoma cells was lower than that of the non-pretreated group (P<0.01).?Conclusions 5-FU could induce apoptosis of gastric carcinoma cells, especially those with the potential of invasion and metastasis. The results suggest that pre-operative chemotherapy may reduce the metastasis and post-operative recurrence of the tumor.
出处
《徐州医学院学报》
CAS
2001年第2期94-96,共3页
Acta Academiae Medicinae Xuzhou
