摘要
目的探讨模拟失重对人胃癌SGC-7901细胞形态、增殖、周期和凋亡的影响。方法采用回转器模拟失重,用图像分析系统软件分析HE染色后SGC-7901细胞形态的改变,用免疫组化法观察细胞增殖细胞核抗原(PCNA)表达的变化,用流式细胞仪测定细胞周期,并用TIJNEL法检测细胞凋亡。结果回转器模拟失重后,回转组SGC-7901细胞12 h、24 h、36 h、48 h及72 h各时间段的细胞面积均较对照组缩小(P<0．01);在36 h时,其长短径之比较对照组增大(P<0．01),其余时间段无明显差异。12 h、24 h和36 h回转组SGC-7901细胞PCNA抗体阳性细胞比率与对照组相比无明显差异,48 h、72 h回转组的PCNA抗体阳性细胞明显减少(P<0．05或P<0．01)。12 h、 24 h和36 h回转组SGC-7901细胞的细胞周期与对照组相比无明显差异,48 h、72 h回转后G0+G1 期细胞显著增多(P<0．01或P<0．05),S+G2+M期细胞则显著减少(P<0．01或P<0．05)。 12 h、24 h、36 h和48 h回转组SGC-7901细胞凋亡比例与对照组相比无明显差异,72 h回转组细胞凋亡比例较对照组明显增多(P<0．01)。结论回转器模拟失重使胃癌SGC-7901细胞的形态发生了变化,48 h、72 h时出现细胞周期阻滞、增殖抑制,72 h时凋亡增加。
Objective To investigate the effects of simulated weightlessness on cellular morphology, proliferation, cell cycle and apoptosis of human gastric carcinoma cell line SGC-7901. Methods Five experimental groups of the cell line were cultured in the rotating clinostat for 12 h, 24 h, 36 h, 48 h and 72 h respectively. Image-pro4.5 image analysis system was employed for morphometric study. The expressions of proliferating cell nuclear antigen （PCNA） were examined by immunohistochemical stain. The changes of cell cycle were examined by a cytometer. Apoptosis was measured by TUNEL method. Results Compared with control group, the area of cell line SGC-7901 of all 5 experimental groups decreased significantly （P〈0. 01）, the ratio of long diameter and short diameter increased significantly in 36 h simulated weightlessness group（P〈0.01）,but did not change significantly in other groups. Compared with control, the expression of PCNA of cell line SGC-7901 did not change significantly in 12 h, 24 h, and 36 h groups, but decreased significantly in 48 h and 72 h groups （P〈0.05 or P〈0.01）. Compared with control, the cell cycles of cell line SGC-7901 in 12 h, 24 h, and 36 h groups did not change significantly, but the cells in Go＋G1 phase in 48 h and 72 h groups increased significantly （P〈0.01 or P〈0. 05） ; accordingly, the cells in S＋G2 ＋M phase decreased significantly （P〈0.0l or P〈0. 05）. Compared with control, the apoptosis index of cell line SGC-7901 in 12 h, 24 h, 36 h and 48 h groups did not change significantly, but increased significantly in 72 h group. Conclusions Simulated weightlessness in a rotating clinostat may lead morphological change of human carcinoma cell line. The conversion of cell from G1 phase to S phase was blocked, the proliferation was inhibited after rotating for 48 h and 72 h, the increased apoptosis index was found after 72 h rotating.
出处
《中华航空航天医学杂志》
CSCD
2005年第4期262-266,F0004,共6页
Chinese Journal of Aerospace Medicine
基金
总装备部预研基金项目(No.51400020204JB1002)
关键词
失重模拟
胃肿瘤
肿瘤细胞
培养的
细胞大小
增殖细胞核抗原
细胞周期
脱噬作用
Weightlessness simulated
Stomach neoplasms
Tumor cells, cultured
Cell size
Proliferating cell nuclear antigen
Cell cycler Apoptosis
