摘要
应用SDS-PAGE和免疫印渍试验(EITB),证明体外培养的细粒棘球蚴生发细胞和囊液含有特异性抗原,分子量为52kDa和38kDa,而囊壁则含有此2种抗原。生发细胞的52kDa抗原可识别感染小鼠和包虫病患者血清的特异性抗体,而对囊虫病人和正常人血清则无反应条带。
Specific antigens are detected in cyst fluid and cyst wall of Echinococcus granulosus, as well as germinal cells cultivated in vitro by sodium dodecyl sulphate polyacrylamide gel elec-trophoresis (SDS-PAGE) and enzyme linked immunotransfer blot technique (ELIB), using the sera from mice infected with protoscoleces of E. granulosus for at least 10 months. A specific reaction band of 52 kDa or 38 kDa was detected in soluble protein of germinal cells and cyst fluid, respectively, but these two reaction bands were present concurrently in the cyst wall. The sera from 7 normal controls, 7 hydatidosis patients and 3 cysticercosis patients were used to study the specific antigen from the germinal cells. The results noted that the reaction band of 52 kDa was seen in all the sera from hydatidosis patients, while the sera from normal human controls and cysticercosis patients showed no reaction band.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1993年第1期63-65,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
卫生部青年科学基金
关键词
棘球属
体外培养
抗原
抗体
ELIB
Echinococcus
germinal cell, in vitro culture
enzyme linked immunotransfer blot technique
antigen, antibody
