摘要
应用SDS-PAGE、ELIB,ELISA等方法,对猪囊尾蚴的水溶性抗原(Ag-W)和尿素溶性抗原(Ag-u)进行对比分析。发现Ag-w和Ag-u分别有14条和9条主带被囊尾蚴病病人血清所识别;有10条和4条主带被包虫病病人血清所识别;有8条和4条主带被华支睾吸虫病患者血清所识别。ELISA检测显示、血清1:400稀释时,囊尾蚴病病人血清(60例)。Ag-w和Ag-u的阳性率分别为86.6％和96.9％,华支睾吸虫病病人血清(24例)和正常人血清均为阴性。包虫病人血清(30例)分别为46.6％和16.6％。表明两种抗原虽有与其它寄生虫产生交叉反应的成份,但Ag-u明显少于Ag-w,Ag-u的敏感性和特异性明显优于Ag-w。用Ag-u代替Ag-w进行囊尾蚴病的免疫学诊断,可提高诊断效价。
Urea-soluble antigens (Ag-u) and water-soluble antigens (Ag-w) of Cysticercus cellu-losae were studied by using ELIB and ELISA, respectively. The results showed that using Ag-u and Ag-w, 9 and 14 bands of polypeptides could be recognized by sera from cysticerco-sis patients; 4 and 10 bands could be recognized by sera from hydatidosis patients; whereas 4 and 8 bands could be recognized by sera from clornochiasis patients.Using ELISA at serum dilutions of 1 : 400, the positive rate of sera against Ag-u and Ag-w was 96. 6% and 86. 6% .respectively in 60 cysticercosis patients; 16. 6% and 46. 6% respectively in 30 hydatidosis patients and negative in 24 clornorchiasis patients and 50 normal healthy persons, indicating that both Ag-u and Ag-w share common antigen components causing cross reaction with the sera from hydatidosis and clonorchiasis patients. However, such components were fewer in Ag-u than in Ag-w. In view of the fact that Ag-u is superior to Ag-w in both sensitivity and specificity, Ag-u might be better used for the diagnosis of cysticercosis.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1993年第1期53-56,共4页
Chinese Journal of Parasitology and Parasitic Diseases
