摘要
应用ELISA双抗体夹心法检测囊虫病患者血清和脑脊液(CSF)中的循环抗原(CA),并对血清和CSF进行了预处理。CSF经沸浴处理后CA阳性率为92. 06％(58/63)。血清用3.5％聚乙二醇(PEG)浓集、1％木瓜酶消化,再作沸浴处理后,阳性率为91.26％(188/206)。血清CA与抗体的总阳性率为97.75％(97/89)。脑囊虫病患者CSF的CA阳性率为88.24％(15/17),血清CA阳性率为94.12％(16/17)。患者经药物治疗后临床症状的变化与血清CA含量的变化一致.虫体死亡后约30d患者血清CA含量明显减少或消失。
Circulating antigen (CA) in serum and cerebrospinal fluid (CSF) of patient with cysticercosis was detected by sandwich ELISA. CA in serum and CSF was combi d into immuno- complex, therefore the positive rate depended on the treatment method of sera of CSF. The posi- tive rate of CSF treated by boiling water bath was 92. 06%(58/63). The positive rate of sera was 91.26%(188/206) after taken the following treatment. IC was separated and concentrated from serum with 3. 5% polyethyleneglycol,digested with 1% papain,then boiled in water bath. The to- tal positive rate of CA and antibody in sera was 97. 75%(87/89). The positive rates of CA in CSF and sera of patients with neurocysticercosis were 88. 24%(15/17) and 94. 12%(16/17)respective- ly. The total positive rate of sera and CSF was 100%(17/17). After patient was treated by medicine, the change of clinical symptom was in accordance with the change of quantity of CA in serum. CA in the serum of the patient was reduced obviously in quantity or disappeared after Cysticercus cellulosae was killed in about 30 days.
出处
《中国寄生虫病防治杂志》
CSCD
1993年第1期16-19,共4页
Chinese Journal of Parasitic Disease Control
