摘要
目的 将激光共聚焦显微镜 (LSCM )引入骨计量学 ,与双能X线骨密度仪 (DEXA)相比较 ,探索骨计量学与生物力学等的关系。方法 选 3个月龄体质量 2 40 g的雌性SD大鼠 15只 ,随机分为卵巢切除组 (8只 )及假手术对照组 (7只 )。饲养 14周后 ,于腹膜下注射四环素 3 0mg/kg ,16周后 ,处死大鼠分离椎骨 ,甲基丙烯酸甲酯包埋 ,采用水磨机制成厚约 2 0 0～ 40 0 μm的切片 ,用激光共聚焦显微镜直接观察。同时 ,进行DEXA等其他检查。结果 去卵巢大鼠骨小梁有明显的改变 ,其中 ,去卵巢大鼠骨小梁面积百分率、骨小梁间隔及骨小梁数目分别为 (17.5± 2 .9) %、(2 88.5± 68.2 ) μm、 (3 .0± 0 .6) /mm较对照组(2 4.0± 4.9) %、(194.5± 2 6.5 ) μm、(3 .9± 0 .3 7) /mm均有显著改变 (P均 <0 .0 5 )。较DEXA更早发现了这种变化 ,将其与骨生物力学结合 ,发现椎骨最大应变与骨皮质厚度呈负相关 (r =-0 .64 65 )。结论 LSCM应用于骨形态计量具有准确、精度高等优点 。
Objective The laser scanning confocal microscope(LSCM) is a new highly distinguishing microscopy.Introduction of LSCM into the bone morphometry aims to find the characteristic of LSCM in bone morphometry.Methods 15 Sprague Dawley female rats, three months old, were divided into two groups in random, A group(8 rats): ovariectomized; B group(7 rats): sham operated. These rats were injected tetracycline (30 mg/kg) under peritoneum after feeding for 14 weeks,and killed after 16 weeks.The vertibras were separated. After these, dehydrated tissues were embeded by methylmethacrlate (MMA). Then they were sectioned at 200-400 μm by water mill. The section was visualized by LSCM. Simultaneously the vertibras were measured by DEXA and others.Results Bone trabecula measure in the ovariectomized rats showed significant changes(All P< 0.05 ),with area percentage,bone trabecula interspace and bone trabecula number( 17.5 ± 2.9 )%,( 288.5 ± 68.2 )μm,( 3.0 ± 0.6 )/mm,while the sham operated as ( 24.0 ± 4.9 )%,( 194.5 ± 26.5 )μm,( 3.9 ± 0.37 )/mm.LSCM detected these changes earlier,and in conjuction with biomechanics,showed a negative correlation between maximum strain and cortical bone thickness (r= -0.6465 ).Conclusion LSCM has advantages of exactness,accuracy and others in morphometry.The change of cortical bone thickness has some important significance for bone fracture due to postmenopausal osteoporosis.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2001年第1期14-16,共3页
Chinese Journal of Physical Medicine and Rehabilitation
