摘要
【目 (的】探讨己酮可可酮碱 (PTX)对非协调性异种心脏移植 [CTx(DC) ]存活时间的影响。【方法】 5 0只雄性豚鼠和 5 0只雄性SD大鼠随机分成A、B、C 3组进行异种心脏 (豚鼠 大鼠 )移植于颈部 ,移植前 1hA组受体生理盐水 1mL腹膜腔内注射 (ip) ,B组受体PTX 5 0mg/kgip ,C组供体、受体均以PTX 5 0mg/kgip。用免疫组织化学方法检测移植心脏血管内皮细胞 (EC)P 选择素表达情况。【结果】①B、C组移植心脏平均存活时间分别为 2 5 6min和 2 5 9min ,较A组 13 9min明显延长 ,P<0 0 5。②移植后 8min和排斥时移植心脏血管ECP 选择素表达B、C组不如A组明显 ,P<0 .0 5。【结论】PTX可以通过保护EC ,延长CTx(DC)
Objective To understand the effect of pentoxifylline (PTX) in xenograft survival of discordant cardiac xenotransplantation [CTx(DC)]. Methods 50 male guinea pigs and 50 male SD rats were randomly divided into three groups. CTx(DC) (guinea pig SD rat) were carried out. The animals accepted management at 1 h pretransplantation as follows: 1 mL physiological saline intraperitoneal (ip) for recipients in group A, 50 mg/kg PTX (ip) for recipients in group B and 50 mg/kg PTX (ip) for both donors and recipients in group C. The P selectin expression of vascular endothelial cells(EC) in cardiac xenografts was detected by immunohistochemical staining. Results ①The mean survival time of cardiac xenografts were 25 6 min (group B) and 25 9 min (group C) was longer than that of group A (13.9 min, P<0.05). ②P selectin expression was lower in group B and group C than in group A at 8 min posttransplantation and rejection (P<0.05). Conclusion PTX may prolong xenograft surivival of CTx(DC) by protecting EC.
出处
《中山医科大学学报》
CSCD
北大核心
2001年第2期100-102,共3页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
广东省卫生厅"五个一科教兴医工程"基金资助项目! (1996年度 )
关键词
己酮可可碱
心脏移植
异种移植
P-选择素
pentoxifylline
heart transplantation
transplantation,heterotogous
P selectin
cell
