摘要
Three wheat varieties different in development properties were treated by means of vernalization,de vernalization and their protein extracts were analyzed by SDS PAGE. The results showed that vernalization and de vernalization treatment not only increased the amount of soluble proteins,but also induced some new proteins (53.2 kDa,46 kDa) in two winter wheat varieties Mercia and Jing 841. The two new proteins also existed in spring wheat,but were absent in winter wheat before vernalization. The peroxidase and esterase isoenzyme activity also increased in the vernalization process, indicating that peroxidase and esterase activity may be required for vernalization of winter wheat. Glucose 6 phosphate isoenzyme activity changes may also be related to vernalization development of winter wheat. However, superoxide dismutase (SOD) isoenzyme activity was not changed by vernalization treatment. These results demonstrated that the vernalization was a process that involved changes in gene expression, protein synthesis and enzyme activities.
Three wheat varieties different in development properties were treated by means of vernalization,de vernalization and their protein extracts were analyzed by SDS PAGE. The results showed that vernalization and de vernalization treatment not only increased the amount of soluble proteins,but also induced some new proteins (53.2 kDa,46 kDa) in two winter wheat varieties Mercia and Jing 841. The two new proteins also existed in spring wheat,but were absent in winter wheat before vernalization. The peroxidase and esterase isoenzyme activity also increased in the vernalization process, indicating that peroxidase and esterase activity may be required for vernalization of winter wheat. Glucose 6 phosphate isoenzyme activity changes may also be related to vernalization development of winter wheat. However, superoxide dismutase (SOD) isoenzyme activity was not changed by vernalization treatment. These results demonstrated that the vernalization was a process that involved changes in gene expression, protein synthesis and enzyme activities.
出处
《遗传》
CAS
CSCD
北大核心
2001年第1期62-62,共1页
Hereditas（Beijing)
