摘要
protoplasts having only 24～28 chromosomes and callus derived Haynaldia villosa potoplasts with 11 14 chromosomes was carried out by a PEG method. A high frequency of putative hybrid calli and plants were regenerated from fusion products. Their hybrid nature was identified by cytological, biochemical and 5S rDNA spacer sequence analysis. The results showed that all the analyzed regenerated calli and plants were hybrids. GISH (genomic in situ hybridization) analysis proved the existence of the chromosomes of both donors and the occurrence of translocation between the chromosomes of the two species in the hybrid clones. The analysis of the cytoplasmic DNA using mitochondrion and chloroplast specific probes revealed that some degree of recombination between the organellar genome of the two species occurred. The gross morphology of hybrid plants resembled that of two donors. One hybrid was fertile and gave rise to seed, which resembled that of Haynaldia villosa in morphology. In conclusion, we generated fertile somatic hybrid by intergeneric somatic hybridization. The co existence of both species’chromosomes in the hybrid clones in relation to their regeneration capacity and the production of fertile hybrid plants will be discussed in the speech.
protoplasts having only 24～28 chromosomes and callus derived Haynaldia villosa potoplasts with 11 14 chromosomes was carried out by a PEG method. A high frequency of putative hybrid calli and plants were regenerated from fusion products. Their hybrid nature was identified by cytological, biochemical and 5S rDNA spacer sequence analysis. The results showed that all the analyzed regenerated calli and plants were hybrids. GISH (genomic in situ hybridization) analysis proved the existence of the chromosomes of both donors and the occurrence of translocation between the chromosomes of the two species in the hybrid clones. The analysis of the cytoplasmic DNA using mitochondrion and chloroplast specific probes revealed that some degree of recombination between the organellar genome of the two species occurred. The gross morphology of hybrid plants resembled that of two donors. One hybrid was fertile and gave rise to seed, which resembled that of Haynaldia villosa in morphology. In conclusion, we generated fertile somatic hybrid by intergeneric somatic hybridization. The co existence of both species’chromosomes in the hybrid clones in relation to their regeneration capacity and the production of fertile hybrid plants will be discussed in the speech.
出处
《遗传》
CAS
CSCD
北大核心
2001年第1期60-61,共2页
Hereditas（Beijing)
