摘要
Rich genetic resources are contained among wheat wild relatives. Cytogenetics Institute of Nanjing Agricultural University commences to transfer these genetic resources from relatives into common wheat. The hybrids between common wheat and Haynaldia villosa, Leymus racemosus,Roegneria ciliaris, Roegneria Kamoji, Secale cereale or Thinopyrum baserabicum, and their alien chromosome stocks such as addition, substitution, translocation and introgression lines were developed via successive pollinations and immature embryo rescues. The resulted progenies were characterized using mitotic and meiotic analysis combining with chromosome banding and in situ hybridization. Isozyme pattern and RFLP analysis were used to determine the homoeologous relationship between alien chromosomes and those of common wheat. Irradiation, Ph system (homoeologous pairing control system), gametocidal chromosome effect were successfully employed to induce translocation and deletion lines. Chromosome C banding, in situ hybridization, RFLP and trait tracing were used to identify alien chromosome, chromosome segments, breakage point of translocation or deletion, and to map the introgressed genes such as those conferring resistance to powdery mildew, yellow rust, Fusarium head blight and take all diseases. As the result of above effort, new genetic materials, which contained multiple useful alien genes, were developed. To improve the agronomic characters of the derived lines, back crosses or “rolling” crosses using superior varieties or lines as recurrent parents are currently being conducted.
Rich genetic resources are contained among wheat wild relatives. Cytogenetics Institute of Nanjing Agricultural University commences to transfer these genetic resources from relatives into common wheat. The hybrids between common wheat and Haynaldia villosa, Leymus racemosus,Roegneria ciliaris, Roegneria Kamoji, Secale cereale or Thinopyrum baserabicum, and their alien chromosome stocks such as addition, substitution, translocation and introgression lines were developed via successive pollinations and immature embryo rescues. The resulted progenies were characterized using mitotic and meiotic analysis combining with chromosome banding and in situ hybridization. Isozyme pattern and RFLP analysis were used to determine the homoeologous relationship between alien chromosomes and those of common wheat. Irradiation, Ph system (homoeologous pairing control system), gametocidal chromosome effect were successfully employed to induce translocation and deletion lines. Chromosome C banding, in situ hybridization, RFLP and trait tracing were used to identify alien chromosome, chromosome segments, breakage point of translocation or deletion, and to map the introgressed genes such as those conferring resistance to powdery mildew, yellow rust, Fusarium head blight and take all diseases. As the result of above effort, new genetic materials, which contained multiple useful alien genes, were developed. To improve the agronomic characters of the derived lines, back crosses or “rolling” crosses using superior varieties or lines as recurrent parents are currently being conducted.
出处
《遗传》
CAS
CSCD
北大核心
2001年第1期59-60,共2页
Hereditas（Beijing)
