摘要
以黄花倒水莲为试材,采用单因素试验和正交实验相结合的方法,研究了超声波频率、超声波功率、提取温度、提取时间、乙醇浓度、料液比对黄花倒水莲总黄酮提取率的影响,以优化超声波提取黄花倒水莲总黄酮的工艺条件。结果表明:超声波提取黄花倒水莲总黄酮最佳提取工艺为超声波频率为高频(61kHz),超声波功率为420 W,提取温度55℃,料液比为1∶15g/mL,乙醇浓度90%,提取时间40min;试验结果表明优选出的提取工艺稳定、重复性好,该试验为黄花倒水莲总黄酮的提取提供了依据。
Taking Polygala fallax as material, using the method of single factor test and orthogonal test,the effect of ultrasonic frequency, ultrasonic power, extracting time, extracting temperature,alcohol concentration and ratio of solid and liquid on extracting rate of flavonoid from Polygala fallax were studied, to optimize extracting technique. The results showed that the optimal extracting conditions were ultrasonic frequency 61 kHz, ultrasonic power 420 W, extracting temperature 55℃ ,the ration of solid and liquid 1 ： 15 g/mL,alcohol concentration 90% ,extracting time 40 rain. The stability and repeatability of this technique were good. It could provide the basis for extracting flavonoid from Polygala fallax.
出处
《北方园艺》
CAS
北大核心
2014年第12期134-137,共4页
Northern Horticulture
基金
广西科技攻关资助项目(桂科攻11107010-1-1)
广西植物功能物质研究与利用重点实验室资助项目(ZRJJ2013-10)
广西自然科学基金资助项目(2013GXNSFBA019054)
广西植物研究所基本业务费资助项目(桂植业12011)
关键词
黄花倒水莲
单因素试验
正交实验
提取工艺
Polygala fallax
single factor test
orthogonal test
extraction technology
