摘要
目的:建立异硫氰酸荧光素(FITC)诱导的Th2型变应性接触性皮炎(Allergic contact dermatitis,ACD)模型,观察升麻素是否具有抑制过敏性炎症的作用并探讨初步的作用机制。方法:BALB/c小鼠在d1、d2天用FITC溶液腹部致敏皮肤2次,同时给药至d5天(诱导相),d6天用FITC溶液攻击小鼠右耳,24h后测定小鼠耳肿胀程度及小鼠两耳重量,取耳组织做耳病理学检查,同时部分耳组织制备匀浆,用ELISA方法检测IL-4、IL-9、IL-13及IFN-γ的水平。结果:升麻素25 mg/kg、50 mg/kg诱导相给药能显著抑制小鼠耳肿胀;耳组织病理学显示,给予升麻素组小鼠局部组织液的渗出、充血、淋巴细胞浸润等炎症状态明显减轻;同时能显著降低小鼠耳组织匀浆中2型细胞因子IL-4、IL-9、IL-13水平,而对IFN-γ水平未见明显影响。结论:升麻素诱导相给药对FITC诱导的小鼠Th2型变应性接触性皮炎有抑制作用,其作用机制可能与调节2型细胞因子有关。
Objective： This study was carried out to investigate the effective and mechanism of cimifugin on type 2 allergic contact dermatitis induced by FITC. Methods： The BALB /c mice were sensitized with FITC on days 1 and 2,on the shaved abdomen skin. On day 6,the animals were challenged on their right ears with FITC. Simultaneously,the mice were administered cimifugin from day 1 to day 5. On day 7,ear swelling was measured and the infiltration of lymphocytic was investigated by hematoxylin and eosin（ H＆E） staining. The levels of IL-4,IL-9,IL-13 and IFN-γ were quantified by ELISA and BCA. Results： The results suggested that mouse ear swelling was dramatically inhibited by25 mg /kg,50 mg /kg cimifugin. Histopathological results indicated that mice given cimifugin has significant improvement on local tissue fluid exudation,congestion,and lymphocytic infiltration was remarkably reduced by cimifugin. At the same time type 2 cytokines IL-4,IL-9,IL-13in the mouse ear tissue homogenate were obviously reduced by cimifugin,while no significant changes with type 1 cytokines IFN-γ. Conclusion： Cimifugin evidently inhibited FITC-induced type 2 allergic contact dermatitis,and its mechanism might related to regulating the balance of Th1 /Th2 by inhibiting type 2 cytokines.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2014年第2期28-30,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金资助81073121、81373549
江苏高校优势学科建设工程资助项目
'青蓝工程'资助
