摘要
目的研究中药复方加味四逆散(JWSNS)分时给药对慢性应激大鼠生物节律的影响。方法采用慢性不可预知轻度应激建立慢性应激大鼠模型。运用余弦节律分析法观察分析慢性应激大鼠昼夜体温节律、血清5-羟色胺(5-hydroxy tryptamine,5-HT)、褪黑素(melatonin,MT)、皮质酮(corticosterone,CORT)分泌及其节律的变化以及JWSNS的影响。血清5-HT、MT、CORT含量测定采用酶联免疫吸附试验。结果 JWSNS 3个给药组对模型大鼠体重增长缓慢现象没有抑制作用,但可以提高模型大鼠糖水偏爱度(P<0.01),表明JWSNS具有抗抑郁的效应。慢性应激使大鼠昼夜体温波动加剧,白昼体温更低,夜间体温更高,并且这种现象在谷值方面表现得更为明显。JWSNS酉时、卯酉分时给药对此现象具有抑制作用(P<0.05,P<0.01),且酉时给药表现为对峰值、谷值的双相调节作用,而卯酉分时给药则主要是作用于光时相,即对谷值(白昼)进行调节。慢性应激大鼠的体温节律出现延迟现象,主要表现为时相的推后,只有JWSNS卯时给药可以对体温节律延迟有抑制作用。慢性应激大鼠昼夜血清5-HT、MT、CORT含量波动幅度减小,分泌节律紊乱,主要表现为时相的大幅提前。JWSNS酉时和卯酉分时给药对大鼠血清5-HT水平低下具有抑制作用(P<0.01);JWSNS酉时给药对血清MT含量波动幅度的减小具有抑制作用(P<0.01),而JWSNS卯时给药和卯酉分时给药则对MT分泌节律紊乱具有抑制作用(P<0.01);JWSNS酉时和卯酉时给药可抑制CORT的升高,对血清CORT含量波动幅度的减小具有抑制作用(P<0.01)。结论 JWSNS卯时给药抗抑郁主要通过调节体温、激素以及神经递质分泌的节律,酉时给药抗抑郁主要通过调节激素和神经递质的分泌量,而卯酉分时给药则具有以上两方面的综合效应。
Aim To study the effects of timeshare ad-ministration of Jiaweisinisan （ JWSNS ） on biological rhythm of rats with chronic stress. Methods Unpre-dictable mild chronic stress was used. Cosine rhythm analysis was adopted to observe and analyze the chan-ges of circadian rhythms of body temperature and se-cretion of 5-hydroxy tryptamine （ 5-HT ） , melatonin （ MT） and corticosterone （ CORT ） and the effects of JWSNS. Serum 5-HT,MT and CORT were detected by enzyme-linked immunosorbent assay. Results Three groups of administration of JWSNS had no inhibitory effects on weight gain in model rats,but improved the sucrose preference （ P 〈0.01 ） , which shows JWSNS has anti-depressant effects. Chronic stress made diurnal temperature volatile in rats,the phenomenon of lower body temperature in the day and higher in the night was more obvious in the valley value, which could be inhibited by administration of JWSNS at 17 ： 30 or at 7： 30 and 17 ： 30 respectively （ P 〈0 . 05 , P 〈0.01 ） . Administration at 17 ： 30 had biphasic regulatory effects on the peak and valley value,while administration at 7 ： 30 and 17 ： 30 respectively mainly acted on the light phase,namely regulated the valley value. Rats with chronic stress showed delays of body temperature rhythm,namely postponed phase, which could only be inhibited by administration at 7 ： 30 . There were de-creases in votatility and disorders in secretion rhythm, as was shown by dramatically accelerated phase of serum 5-HT, MT and CORT in rats with chronic stress. Administration of JWSNS at 17 ： 30 or at 7 ： 30 and 17 ： 30 respectively had inhibitory effects on lower ser-um level of 5-HT in model rats （ P 〈0.01 ） . Adminis-tration of JWSNS at 17 ： 30 could inhibit the decreases in volatility of serum MT（P〈0. 01）,while administra-tion of JWSNS at 7 ： 30 or at 7 ： 30 and 17 ： 30 re-spectively could inhibit the secretion rhythm disorders （ P〈0.01 ） . Administration of JWSNS at 17 ： 30 or at 7 ： 30 and 17 ： 30 respectively could inhibit the CORT increase and the decreases in volatility. ConclusionAdministration of JWSNS at 7 ： 30 achieves anti-de-pressant effects by regulating rhythms of hormones and neurotransmitters. Administration of JWSNS at 17 ： 30 achieves anti-depressant effects by adjusting the secre-tion of hormones and neurotransmitters,while administration of JWSNS at 7 ： 30 and 17 ： 30 respectively achieves anti-depressant effects by those two above-men-tioned aspects.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2014年第6期867-874,共8页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 30873389)
关键词
应激
抑郁症
5-羟色胺
褪黑素
皮质酮
加味四逆散
stress
depression
5-hydroxy tryptamine
melatonin
corticosterone
Jiaweisinisan
