摘要
The traditional Chinese medicine Jiaweisinisan has antidepressant effects,and can inhibit hypothalamus-pituitary-adrenal gland axis hyperactivity in stress-induced depression.In this study,rat hippocampal neural precursor cells were cultured in serum-free medium in vitro and a stress damage model was established with 120 IJM corticosterone.Cells were treated with 10%(v/v)Jiaweisinisan drug-containing serum and the corticosterone antagonist RU38486.Results of the 3-(4,5-dimethylthiazol-2-yl)-3,5-di-phenytetrazoliumromide assay showed that both Jiaweisinisan drug-containing serum and RU38486 promoted the proliferation of neural precursor cells after corticosterone exposure.Immunofluorescence detection showed that after Jiaweisinisan drug-containing serum and RU38486 treatment,the 5-bromo-2-deoxyuridine/terminal deoxynucleotidyl transferase dUTP nick end labeling ratio in hippocampal neural precursor cells significantly increased,and the apoptotic rates of glial cells reduced,and neuron-like cell differentiation from neural precursor cells significantly increased.Our experimental findings indicate that Jiaweisinisan promotes hippocampal neurogenesis after stress damage.
基金
supported by the National NaturalScience Foundation of China,No.30500660
