摘要
采用Clark型氧电极法研究松属素对柑橘青霉病菌(Penicillium italicum)菌丝体呼吸作用的影响,结果表明:松属素处理对柑橘青霉病菌菌丝体呼吸具有显著的抑制作用,且抑制效应呈显著的浓度效应。随着松属素处理时间的延长,菌丝体呼吸作用的抑制效应也随之增强,当松属素处理菌丝体时间延长至2h时,松属素对菌丝体呼吸作用的抑制率由最初的53.37%增至84.35%。松属素对幼嫩菌丝体(培养12h)的呼吸抑制效果最显著,呼吸抑制率达78.97%;随着菌龄的增加,松属素对菌丝体呼吸的抑制作用下降,对培养60h的菌丝体呼吸抑制率仅为29.25%。进一步研究表明,松属素在较低浓度下即对柑橘青霉病菌线粒体呼吸表现出显著的抑制作用。通过比较呼吸电子传递链典型呼吸抑制剂(抗霉素A、鱼藤酮、水杨羟肟酸和叠氮化钠)对松属素抑制呼吸的叠加率,发现松属素与鱼藤酮的叠加率最低,其抑制柑橘青霉病菌呼吸作用部位可能在线粒体呼吸电子传递链上NADH向辅酶Q的传递过程。
The effects of pinocembrin on the mycelia respiration of citrus blue mold（Penicilliurn italicurn） were studied by clark oxygen electrode. The results showed that pinocembrin treatment signif- icantly inhibited the myeelia respiration of P. italicurn in a concentration-dependent manner. The inhibi- tory effect of mycelial respiration increased with the prolonging of pinocembrin treatment. When the treatment time was extended to 2 h,the inhibition rate was changed from 53. 37 % to 84. 35%. The inhibitory effect of pinocembrin on the respiration was most significant for young mycelia （cultured 12 h）, and the inhibitory rate was up to 78. 97%. With the increment of cell age, the inhibitory effect of pino- cembrin on mycelia respiration declined. The respiration inhibitory rate of myeelia cultured for 60 h was only 29.25%. Results showed that pinocembrin treatment had an obvious inhibition on the mitochondria respiration of P. italicum at low concentration. Comparing synergistic inhibition of the typical respiratory inhibitors （antimyein A, rotenone, salicyl hydroxamie acid and sodium azide） and pinocembrin on the res- piration of P. italicurn,the results showed that pinocembrin and rotenone had the same pathway on the myeelia respiration of P. italicurn. Therefore,pinoeembrin had a significant inhibitory effect on P. Italicure by affecting respiration metabolism.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2014年第4期119-123,共5页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(31271969)
关键词
松属素
柑橘青霉病菌
菌丝呼吸
抑制作用
线粒体
pinocembrin
citrus blue mold （Penicillium italicum）
mycelia respiration
inhibition mitochondria
