摘要
目的检测微小RNA（miRNA，miR）-500在丙型肝炎肝硬化到肝癌病程中的动态表达，探讨miR-500表达变化在丙型肝炎肝硬化再到丙型肝炎相关性肝癌病变过程中的作用机制。方法选取本院收治8例丙型肝炎肝硬化患者为肝硬化组，8例丙型肝炎病毒感染相关性肝癌患者为肝癌组，另选取8名健康体检者为对照组，利用实时定量聚合酶链反应（Real—timePCR）法测定上述3组人群血浆中miR-500的含量。结果肝硬化组中血浆miR-500表达水平为5．72±2．28，高于对照组的0．95±0．48，两者差异有统计学意义（P〈0．05）；肝癌组患者血浆中miR-500表达水平为18．62±8．13，高于对照组和肝硬化组，两者差异有统计学意义（P〈0．05）。结论miR-500可能参与内型肝炎肝硬化到肝癌的发生发展过程。
Objective To investigate the dysregulation of microRNA-500 （miR-500） in the development process from hepatitis C liver cirrhosis to hepatocellular carcinoma so as to clarify the role of change of miR-500 expression in the development process from the hepatitis C to liver cirrhosis and hepatocellular carcinoma. Methods From Nov. 2012 to Oct. 2013, 8 cases of hepatitis C liver cirrhosis and 8 cases of hepatitis C virus infection-related liver cancer were selected as the research objects, and 8 cases of the healthy check-up served as the control group. The expression level of miR-500 in the plasma of the above three groups was tested by using real-time quantitative polymerase chain reaction （Real-time PCR）. Resuits The expression level of miR-500 in liver cirrhosis group was 5.72 ± 2. 28, higher than 0. 95 ± 0. 48 in the control group, and that in liver cancer group was 18.62 ± 8.13, higher than in the control group and liver cirrhosis group. Conclusion The miR-500 may be involved in the development process from chronic hepatitis C liver cirrhosis to liver cancer.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第6期1316-1317,共2页
Chinese Journal of Experimental Surgery
